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首页> 外文期刊>Journal of Biophotonics >Development of a polarization imaging method to detect paraffin-embedded pathology tissues before applying other techniques
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Development of a polarization imaging method to detect paraffin-embedded pathology tissues before applying other techniques

机译：在应用其他技术之前，在涂覆石蜡包埋病理组织中偏振成像方法的发展

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摘要

The present article describes the development of a technique, applied to paraffin-embedded tissues, which uses three different wavelengths of monochromatic light (lambda 1 = 445 nm, lambda 2 = 540 nm and lambda 3 = 660 nm) for the measures of the degree of polarization, degree of linear polarization, degree of circular polarization and birefringence, all obtained from measurements of Stokes parameters by using polarized light. The goal of this study was to detect changes in developing embryonic mouse eye when pregnant mice fed diets without folic acid for variable periods compared with a healthy control group. We present a biomedical diagnostic technique based on polarized light detection applied to paraffin-embedded tissues to visualize the structural damage to aid us in the diagnosis before applying other techniques. Through this method, we can visualize and identify which parts of the tissue were altered with respect to the control group.
机译：本文描述了一种应用于石蜡包埋组织的技术的发展，该技术使用三种不同波长的单色光（λ1=445 nm、λ2=540 nm和λ3=660 nm）测量偏振度、线偏振度、圆偏振度和双折射，所有这些都是通过使用偏振光测量斯托克斯参数获得的。本研究的目的是检测与健康对照组相比，怀孕小鼠在不同时期食用不含叶酸的饮食时胚胎小鼠眼睛发育的变化。我们提出了一种基于偏振光检测的生物医学诊断技术，应用于石蜡包埋组织，以可视化结构损伤，在应用其他技术之前帮助我们进行诊断。通过这种方法，我们可以可视化并识别与对照组相比，组织的哪些部位发生了改变。

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