Instant polarized light microscopy for imaging collagen microarchitecture and dynamics

Yang Bin; Lee Po-Yi; Hua Yi; Brazile Bryn; Waxman Susannah; Ji Fengting; Zhu Ziyi; Sigal Ian A.

首页> 外文期刊>Journal of Biophotonics >Instant polarized light microscopy for imaging collagen microarchitecture and dynamics

【24h】

Instant polarized light microscopy for imaging collagen microarchitecture and dynamics

机译：瞬间偏振光显微镜，用于成像胶原蛋白微体系结构和动力学

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Collagen fibers are a primary load-bearing component of connective tissues and are therefore central to tissue biomechanics and pathophysiology. Understanding collagen architecture and behavior under dynamic loading requires a quantitative imaging technique with simultaneously high spatial and temporal resolutions. Suitable techniques are thus rare and often inaccessible. In this study, we present instant polarized light microscopy (IPOL), in which a single snapshot image encodes information on fiber orientation and retardance, thus fulfilling the requirement. We utilized both simulation and experimental data from collagenous tissues of chicken tendon, sheep eye, and porcine heart to evaluate the effectiveness of IPOL as a quantitative imaging technique. We demonstrate that IPOL allows quantitative characterization of micron-scale collagen fiber architecture at full camera frame rates (156 frames/second herein).

机译：胶原纤维是结缔组织的主要承重成分，因此是组织生物力学和病理生理学的核心。了解胶原在动态载荷下的结构和行为需要同时具有高空间和时间分辨率的定量成像技术。因此，合适的技术非常罕见，而且往往难以获得。在这项研究中，我们提出了即时偏振光显微镜（IPOL），其中单个快照图像编码有关光纤方向和延迟的信息，从而满足要求。我们利用来自鸡腱、羊眼和猪心的胶原组织的模拟和实验数据来评估IPOL作为定量成像技术的有效性。我们证明，IPOL可以在全帧速率（本文中为156帧/秒）下对微米级胶原纤维结构进行定量表征。

著录项

来源
《Journal of Biophotonics》 |2021年第2期|共11页
作者
Yang Bin; Lee Po-Yi; Hua Yi; Brazile Bryn; Waxman Susannah; Ji Fengting; Zhu Ziyi; Sigal Ian A.;
展开▼
作者单位

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

Univ Pittsburgh Sch Med Dept Ophthalmol Pittsburgh PA 15261 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物物理学;
关键词
biomechanics; collagen; deformation; polarized light microscopy;

机译：生物力学;胶原蛋白;变形;偏振光显微镜;

相似文献

外文文献
中文文献
专利

1. Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus [J] . Ying-xian Ma, Chayanis Sutcharitchan, Xiao-die Li, 中草药：英文版 . 2020,第004期
2. Collagen Fibers in Crocodile Skin and Teeth: A Morphological Comparison Using Light and Scanning Electron Microscopy [J] . Piotr Krzysztof Szewczyk, Urszula Stachewicz 仿生工程学报（英文版） . 2020,第004期
3. Polarized Light Microscopy and Spectroscopy of Individual Single-Walled Carbon Nanotubes [J] . Jacques Lefebvre, Paul Finnie 纳米研究：英文版 . 2011,第008期
4. Structural Domain Imaging and Direct Determination of Crystallographic Orientation in Noncentrosymmetric CaaRu2O7 Using Polarized Light Reflectance [J] . Guoxiong Tang, Chao Yao, Yueshen Wu, 中国物理快报：英文版 . 2020,第010期
5. Grazing incidence polarized light imaging of footwear prints [J] . Xin-yi BI, Rui-fang HAN, Ran LIAO, 浙江大学学报（英文版）（C辑：计算机与电子） . 2019,第011期
6. Automatic Evaluation of Collagen Fiber Directions from Polarized Light Microscopy Images [J] . Novak Kamil, Polzer Stanislav, Tichy Michal, Microscopy and microanalysis: The official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada . 2015,第4期

机译：从偏振光显微镜图像自动评估胶原纤维的方向
7. Collagen architecture in the third dimension:3D polarized light microscopy (3DPLM) for mapping in-plane (IP) and out-of-plane (OOP) collagen fiber architecture [J] . Yang Bin, Jan Ning-Jiun, Lam Po, Investigative ophthalmology & visual science . 2017,第8期

机译：胶原型架构在第三尺寸：3D偏振光显微镜（3DPLM），用于在平面内（IP）和外平面（OOP）胶原纤维架构
8. Collagen architecture in the third dimension:3D polarized light microscopy (3DPLM) for mapping in-plane (IP) and out-of-plane (OOP) collagen fiber architecture [J] . Yang Bin, Jan Ning-Jiun, Lam Po, Investigative ophthalmology & visual science . 2017,第8期

机译：胶原型架构在第三尺寸：3D偏振光显微镜（3DPLM），用于在平面内（IP）和外平面（OOP）胶原纤维架构
9. Instant polarized light microscopy for real-time wide-field visualization of collagen architecture [C] . Po-Yi Lee, Bin Yang, Yi Hua, International Conference on Label-free Biomedical Imaging and Sensing . 2020

机译：胶原型架构的实时宽场可视化即时偏振光显微镜
10. Imaging of a model plastic fat system by 3-dimensional wide-field transmitted polarized light microscopy and image deconvolution. [D] . Litwinenko, Jerrold W. 2004

机译：通过3D广角透射偏振光显微镜和图像反卷积对模型塑料脂肪系统进行成像。
11. Imaging of Scleral Collagen Deformation Using Combined Confocal Raman Microspectroscopy and Polarized Light Microscopy Techniques [O] . Nilay Chakraborty, Mian Wang, Jason Solocinski, -1

机译：使用共聚焦拉曼光谱和偏振光显微镜技术对巩膜胶原蛋白变形进行成像
12. Imaging of Scleral Collagen Deformation Using Combined Confocal Raman Microspectroscopy and Polarized Light Microscopy Techniques. [O] . Nilay Chakraborty, Mian Wang, Jason Solocinski, 2016

机译：使用组合共焦拉曼显微光谱和偏振光显微镜技术成像巩膜胶原变形。
13. Image contrast and phase modulated light methods in polarization and inteference microscopy. Instruction manual for the PAR EPM-1 photoelectric polarizing and interference microscope [R] . Allen, R. D. 1964

机译：偏振和干涉显微镜中的图像对比和相位调制光方法。 paR Epm-1光电偏振和干涉显微镜使用说明书

Instant polarized light microscopy for imaging collagen microarchitecture and dynamics

摘要

著录项

相似文献

相关主题

期刊订阅