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首页> 外文期刊>Journal of Biophotonics >Near-infrared double-illumination optical-resolution photoacoustic microscopy
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Near-infrared double-illumination optical-resolution photoacoustic microscopy

机译:近红外双照射光学分辨率光声显微镜

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摘要

Label-free chemical bond imaging is of great importance in biology and medicine. Photoacoustic imaging at the third near-infrared windows (1600-1870 nm, near-infrared-III) provides a stable molecular vibrational imaging tool for lipid-rich tissue owing to the first overtone transition of the C-H bond at 1.7 mu m. However, lacking high-energy pulsed laser sources at 1.7 mu m and the strong water absorption significantly limit the signal-to-noise ratio of the lipid imaging, especially for thin lipid tissues. To circumvent this barrier, we develop near-infrared-III double-illumination optical-resolution photoacoustic microscopy (DIOR-PAM) for improving the sensitivity of label-free lipid imaging. Using the same laser, DIOR-PAM can enhance the sensitivity by nearly 100%, which we prove in the Monte Carlo simulation. We experimentally demonstrated 50% similar to 100% sensitivity enhancements on nonbiological and biological lipid-rich samples.
机译:无标记化学键成像在生物学和医学中具有重要意义。第三个近红外窗口(1600-1870 nm,近红外III)的光声成像为富含脂质的组织提供了稳定的分子振动成像工具,因为在1.7μm处C-H键的第一泛音跃迁。然而,在1.7μm处缺乏高能脉冲激光源和强吸水性显著限制了脂质成像的信噪比,尤其是薄脂肪组织。为了绕过这一障碍,我们开发了近红外III型双照明光学分辨率光声显微镜(DIOR-PAM),以提高无标记脂质成像的灵敏度。使用相同的激光,DIOR-PAM可以将灵敏度提高近100%,我们在蒙特卡罗模拟中证明了这一点。我们在实验中证明,在非生物性和生物性富含脂质的样品上,灵敏度提高了50%,与100%相似。

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