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首页> 外文期刊>Journal of Biophotonics >Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets
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Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets

机译：缺氧诱导胰岛损伤的荧光寿命代谢映射

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摘要

Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting beta-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stressed by a hypoxic environment and immune reactions that cause poor engraftment and graft failure. The current standards to assess islet quality before transplantation are destructive procedures, performed on a small islet population that does not reflect the heterogeneity of large isolated islet batches. In this study, we incorporated fluorescence lifetime imaging microscopy (FLIM) into a pancreas-on-chip system to establish a protocol to noninvasively assess the viability and functionality of pancreatic beta-cells in a three-dimensional in vitro model (= pseudo-islets). We demonstrate how (pre-) hypoxic beta-cell-composed pseudo-islets can be discriminated from healthy functional pseudo-islets according to their FLIM-based metabolic profiles. The use of FLIM during the pretransplantation pancreatic islet selection process has the potential to improve the outcome of beta-cell islet transplantation.
机译：从供体胰腺中分离胰岛是移植分泌胰岛素的β细胞治疗1型糖尿病的必要步骤。然而，这一过程会损害胰岛基底膜，从而导致胰岛功能障碍或死亡。移植后，缺氧环境和免疫反应会进一步加重胰岛的压力，导致移植不良和移植失败。目前在移植前评估胰岛质量的标准是破坏性程序，在一个小的胰岛群体上进行，不能反映大的分离胰岛批次的异质性。在这项研究中，我们将荧光寿命成像显微镜（FLIM）纳入胰腺芯片系统，以建立一个协议，在三维体外模型（=伪胰岛）中无创评估胰岛β细胞的活性和功能。我们展示了（预）缺氧β细胞组成的假胰岛如何根据其基于FLIM的代谢谱与健康功能性假胰岛进行区分。在移植前胰岛选择过程中使用FLIM有可能改善β细胞胰岛移植的结果。

著录项

  • 来源
    《Journal of Biophotonics》 |2020年第12期|共13页
  • 作者

    Zbinden Aline; Carvajal Berrio Daniel A.; Urbanczyk Max; Layland Shannon L.; Bosch Mariella; Fliri Sandro; Lu Chuan-en; Jeyagaran Abiramy; Loskill Peter; Duffy Garry P.; Schenke-Layland Katja;

  • 作者单位

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

    Natl Univ Ireland Anat &

    Regenerat Med Inst Sch Med Coll Med Nursing &

    Hlth Sci Galway Ireland;

    Eberhard Karls Univ Tubingen Res Inst Womens Hlth Dept Womens Hlth Tubingen Germany;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物物理学;
  • 关键词

    FLIM; hypoxia; insulin; pancreatic islet; type 1 diabetes;

    机译：flim;缺氧;胰岛素;胰岛胰岛;1型糖尿病;

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