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Identification and in silico expression analysis of three transcription factors in the wild potato

机译:野马铃薯三种转录因子的鉴定和硅表达分析

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Transcription factors (TFs) are proteins in DNA that regulate the expression of nearby genes by interacting with cis-elements in regulatory regions, such as promoters and enhancers. Transcription factors play an important role in many biological functions, such as stress response, development, morphogenesis, and energy metabolism. The response to stress developed in organisms is created by the complex interaction of many genes at the transcriptional level, and at that stage, many TFs are involved. Aswild plants have a much higher level of genetic variation than their cultivated relatives, they possess qualities to adapt to different conditions, such as stress. Wild species possessing a high level of tolerance to stress conditions have high potential to be used in studies to increase product efficiency. Therefore, it is important to understand the genome structure of wild species. In this study, transcription factors, such as WRKY (W: tryptophan, R: arginine, K: lysine, and Y: tyrosine), basic leucine zipper (bZIP), and NAC (NAM No apical meristem - ATAF1-2 Arabidopsis thaliana activating factor - CUC2 cup-shaped cotyledon), which play an important role in response to stress, were identified in the genome of wild potato (Solarium commersonii) using bioinformatic approaches. The expression levels of these TFs were determined by in silico analyses using two existing transcription libraries. In addition, by comparing the data obtained in this study with the data from the domesticated potato (Solanumtuberosum), WRKY, bZIP, and NAC gene families were evaluated from the evolutionary standpoint.
机译:转录因子(Transcription factors,TFs)是DNA中的蛋白质,通过与调控区的顺式元件(如启动子和增强子)相互作用来调节附近基因的表达。转录因子在应激反应、发育、形态发生和能量代谢等许多生物学功能中发挥着重要作用。生物体对应激的反应是由许多基因在转录水平上的复杂相互作用产生的,在这一阶段,许多转录因子参与其中。由于野生植物的遗传变异水平比栽培的近缘植物高得多,因此它们具有适应不同条件的品质,例如压力。对压力条件具有高度耐受性的野生物种在提高产品效率的研究中具有很大的潜力。因此,了解野生物种的基因组结构非常重要。在这项研究中,转录因子,如WRKY(W:色氨酸,R:精氨酸,K:赖氨酸和Y:酪氨酸),碱性亮氨酸拉链(bZIP)和NAC(NAM No顶端分生组织-ATAF1-2拟南芥激活因子-CUC2杯状子叶),在应激反应中发挥重要作用,利用生物信息学方法,在野生马铃薯(Solarium commersonii)基因组中鉴定了这些基因。这些转录因子的表达水平通过使用两个现有转录文库的电子分析来确定。此外,通过将本研究获得的数据与来自驯化马铃薯(Solanumtuberosum)的数据进行比较,从进化角度对WRKY、bZIP和NAC基因家族进行了评估。

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