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首页> 外文期刊>Journal of Experimental Botany >A barley UDP-glucosyltransferase inactivates nivalenol and provides Fusarium Head Blight resistance in transgenic wheat
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A barley UDP-glucosyltransferase inactivates nivalenol and provides Fusarium Head Blight resistance in transgenic wheat

机译：大麦UDP-葡糖基转移酶灭活Nimalenol，并在转基因小麦中提供镰刀菌长抗抗性

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摘要

Fusarium Head Blight is a disease of cereal crops that causes severe yield losses and mycotoxin contamination of grain. The main causal pathogen, Fusarium graminearum, produces the trichothecene toxins deoxynivalenol or nivalenol as virulence factors. Nivalenol-producing isolates are most prevalent in Asia but co-exist with deoxynivalenol producers in lower frequency in North America and Europe. Previous studies identified a barley UDP-glucosyltransferase, HvUGT13248, that efficiently detoxifies deoxynivalenol, and when expressed in transgenic wheat results in high levels of type II resistance against deoxynivalenol-producing F. graminearum. Here we show that HvUGT13248 is also capable of converting nivalenol into the non-toxic nivalenol-3-O-beta-d-glucoside. We describe the enzymatic preparation of a nivalenol-glucoside standard and its use in development of an analytical method to detect the nivalenol-glucoside conjugate. Recombinant Escherichia coli expressing HvUGT13248 glycosylates nivalenol more efficiently than deoxynivalenol. Overexpression in yeast, Arabidopsis thaliana, and wheat leads to increased nivalenol resistance. Increased ability to convert nivalenol to nivalenol-glucoside was observed in transgenic wheat, which also exhibits type II resistance to a nivalenol-producing F. graminearum strain. Our results demonstrate the HvUGT13248 can act to detoxify deoxynivalenol and nivalenol and provide resistance to deoxynivalenol-and nivalenol-producing Fusarium.
机译：镰刀菌赤霉病是一种禾谷类作物病害，会造成严重的产量损失和谷物霉菌毒素污染。主要致病病原体禾谷镰刀菌（Fusarium graminearum）产生毛霉烯毒素脱氧雪腐镰刀菌烯醇（deoxynivalenol）或尼瓦醇（nivalenol）作为毒力因子。尼瓦醇产生菌在亚洲最为普遍，但在北美和欧洲与脱氧雪腐镰刀菌烯醇产生菌共存的频率较低。之前的研究发现，大麦UDP葡萄糖基转移酶HvUGT13248能有效地解毒脱氧雪腐镰刀菌烯醇，当在转基因小麦中表达时，会导致对产生脱氧雪腐镰刀菌烯醇的禾谷镰刀菌产生高水平的II型抗性。在这里，我们表明HvUGT13248还能够将尼瓦醇转化为无毒的尼瓦醇-3-O-β-d-葡萄糖苷。我们描述了尼瓦醇葡萄糖苷标准物的酶制剂及其在检测尼瓦醇葡萄糖苷结合物的分析方法开发中的应用。表达HvUGT13248糖基化尼瓦醇的重组大肠杆菌比脱氧雪腐镰刀菌烯醇更有效。酵母、拟南芥和小麦中的过度表达导致尼瓦醇抗性增强。在转基因小麦中观察到尼瓦醇转化为尼瓦醇葡萄糖苷的能力增强，这也对产生尼瓦醇的禾谷镰刀菌菌株表现出II型抗性。我们的研究结果表明，HvUGT13248能够解毒脱氧雪腐镰刀菌烯醇和尼瓦醇，并对产生脱氧雪腐镰刀菌烯醇和尼瓦醇的镰刀菌产生抗性。

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