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首页> 外文期刊>Journal of Experimental Botany >Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures
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Ethephon-induced changes in antioxidants and phenolic compounds in anthocyanin-producing black carrot hairy root cultures

机译：Ethephon-诱导抗氧化剂和酚类化合物的抗氧化剂和酚醛化合物的变化，产生的黑胡萝卜毛发根培养物

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摘要

Hairy root (HR) cultures are quickly evolving as a fundamental research tool and as a bio-based production system for secondary metabolites. In this study, an efficient protocol for establishment and elicitation of anthocyanin-producing HR cultures from black carrot was established. Taproot and hypocotyl explants of four carrot cultivars were transformed using wild-type Rhizobium rhizogenes. HR growth performance on plates was monitored to identify three fast-growing HR lines, two originating from root explants (lines NB-R and 43-R) and one from a hypocotyl explant (line 43-H). The HR biomass accumulated 25- to 30-fold in liquid media over a 4 week period. Nine anthocyanins and 24 hydroxycinnamic acid derivatives were identified and monitored using UPLC-PDA-TOF during HR growth. Adding ethephon, an ethylene-releasing compound, to the HR culture substantially increased the anthocyanin content by up to 82% in line 43-R and hydroxycinnamic acid concentrations by >20% in line NB-R. Moreover, the activities of superoxide dismutase and glutathione S-transferase increased in the HRs in response to ethephon, which could be related to the functionality and compartmentalization of anthocyanins. These findings present black carrot HR cultures as a platform for the in vitro production of anthocyanins and antioxidants, and provide new insight into the regulation of secondary metabolism in black carrot.
机译：毛状根（HR）培养物作为一种基础研究工具和次级代谢产物的生物生产系统正在迅速发展。本研究建立了一种从黑胡萝卜中建立和诱导产生花青素的HR培养基的有效方法。利用野生型根瘤菌基因对四个胡萝卜品种的直根和下胚轴外植体进行了转化。在平板上监测HR生长性能，以确定三个快速生长的HR系，两个来自根外植体（系NB-R和43-R），一个来自下胚轴外植体（系43-H）。在4周的时间里，HR生物量在液体介质中积累了25到30倍。在HR生长期间，使用UPLC-PDA-TOF对9种花青素和24种羟基肉桂酸衍生物进行了鉴定和监测。向HR培养物中添加乙烯释放化合物乙烯利，可显著增加43-R线中花青素含量高达82%，NB-R线中羟基肉桂酸浓度>20%。此外，HRs中超氧化物歧化酶和谷胱甘肽S-转移酶的活性因乙烯利而增加，这可能与花青素的功能和区域化有关。这些发现将黑胡萝卜HR培养物作为体外产生花青素和抗氧化剂的平台，并为黑胡萝卜次生代谢的调节提供了新的见解。

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