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首页> 外文期刊>Journal of Fish Biology >Modulation of hepatic miRNA expression in Atlantic salmon (Salmo salar) by family background and dietary fatty acid composition
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Modulation of hepatic miRNA expression in Atlantic salmon (Salmo salar) by family background and dietary fatty acid composition

机译：家庭背景和膳食脂肪酸组成的大西洋三文鱼（沙摩酱）中肝miRNA表达的调节

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摘要

This study finds significant differences in hepatic fatty acid composition between four groups of Atlantic salmon (Salmo salar) consisting of offspring from families selected for high and low capacities to express the delta 6 desaturase isomer b and fed diets with 10% or 75% fish oil. The results demonstrated that hepatic lipid metabolism was affected by experimental conditions (diet/family). The fatty acid composition in the four groups mirrored the differences in dietary composition, but it was also associated with the family groups. Small RNA sequencing followed by RT-qPCR identified 12 differentially expressed microRNAs (DE miRNAs), with expression associated with family groups (miR-146 family members, miR-200b, miR-214, miR-221, miR-125, miR-135, miR-137, miR_nov_1), diets (miR-203, miR-462) or both conditions. All the conserved DE miRNAs have been reported as associated with lipid metabolism in other vertebrates. In silico predictions revealed 37 lipid metabolism pathway genes, including desaturases, transcription factors and key enzymes in the synthesis pathways as putative targets (e.g., srebp-1 and 2, Delta 6fad_b and c, hmdh, elovl4 and 5b, cdc42). RT-qPCR analysis of selected target genes showed expression changes that were associated with diet and with family groups (d5fad, d6fad_a, srebp-1). There was a reciprocal difference in the abundance of ssa-miR-203a-3p and srebp-1 in one group comparison, whereas other predicted targets did not reveal any evidence of being negatively regulated by degradation. More experimental studies are needed to validate and fully understand the predicted interactions and how the DE miRNAs may participate in the regulation of hepatic lipid metabolism.
机译：这项研究发现，四组大西洋鲑鱼（Salmo salar）的肝脏脂肪酸组成存在显著差异，这四组鲑鱼的后代来自选择表达δ6去饱和酶异构体b的高和低能力的家族，并且喂食10%或75%的鱼油。结果表明，肝脏脂质代谢受实验条件（饮食/家庭）的影响。四组中的脂肪酸组成反映了饮食组成的差异，但也与家庭组有关。小RNA测序和RT-qPCR鉴定了12种差异表达的微RNA（DE miRNA），其表达与家族组（miR-146家族成员、miR-200b、miR-214、miR-221、miR-125、miR-135、miR-137、miR-nov___1）、饮食（miR-203、miR-462）或这两种情况有关。在其他脊椎动物中，所有保守的去miRNA都被报道与脂质代谢有关。电子预测显示37个脂质代谢途径基因，包括去饱和酶、转录因子和合成途径中的关键酶，作为假定的靶点（例如srebp-1和2、δ6fad_b和c、hmdh、elovl4和5b、cdc42）。对选定目标基因的RT-qPCR分析显示，表达变化与饮食和家族组（d5fad、d6fad_a、srebp-1）有关。在一组比较中，ssa-miR-203a-3p和srebp-1的丰度存在互惠差异，而其他预测靶点未显示任何证据表明其受到降解的负调节。需要更多的实验研究来验证和充分理解预测的相互作用，以及去miRNA如何参与肝脏脂质代谢的调节。

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