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首页> 外文期刊>Journal of genetics >Mining genes associated with furanocoumarin biosynthesis in an endangered medicinal plant, Glehnia littoralis
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Mining genes associated with furanocoumarin biosynthesis in an endangered medicinal plant, Glehnia littoralis

机译：Glehnia Littoralis在濒临灭绝的药用植物中与Furanocoumarin生物合成相关的采矿基因

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摘要

The endangered medicinal plant Glehnia littoralis is one of the important natural source of furanocoumarin, which has been used as mucolytic, antitussive, antitumour and antibacterial. However, the genetic information of furanocoumarin biosynthesis in G. littoralis is scarce at present. The objective of this study was to mine the putative candidate genes involved in the biosynthesis pathway of furanocoumarin and provide references for gene identification, and functional genomics of G. littoralis. We carried out the transcriptome analysis of leaves and roots in G. littoralis, which provided a dataset for gene mining. Psoralen, imperatorin and isoimperatorin were detected in G. littoralis by high performance liquid chromatography analysis. Candidate key genes were mined based on the annotations and local BLAST with homologous sequences using BioEdit software. The relative expression of genes was analysed using quantitative real-time polymerase chain reaction. Further, the CYP450 genes were mined using phylogenetic analyses using MEGA 6.0 software. A total of 156,949 unigenes were generated, of which 9021 were differentially-expressed between leaves and roots. A total of 82 unigenes encoding eight enzymes in furanocoumarin biosynthetic pathway were first obtained. Seven genes that encoded key enzymes in the downstream furanocoumarin biosynthetic pathway and expressed more in roots than leaves were screened. Twenty-six candidate CYP450 unigenes expressed abundantly in roots and were chiefly concentrated in CYP71, CYP85 and CYP72 clans. Finally, we filtered 102 differentially expressed transcription factors (TFs) unigenes. The transcriptome of G. littoralis was characterized which would help to elucidate the furanocoumarin biosynthetic pathway in G. littoralis and provide an invaluable resource for further study of furanocoumarin.
机译：濒危药用植物北沙参（Glehnia littoralis）是呋喃香豆素的重要天然来源之一，具有溶粘液、镇咳、抗肿瘤和抗菌作用。然而，目前关于沿海革兰中呋喃香豆素生物合成的遗传信息很少。本研究的目的是挖掘可能参与呋喃香豆素生物合成途径的候选基因，并为沿岸革兰菌的基因鉴定和功能基因组学提供参考。我们进行了滨藜叶和根的转录组分析，为基因挖掘提供了数据集。采用高效液相色谱分析法，在滨海克雷伯菌中检测到补骨脂素、欧前胡素和异欧前胡素。利用BioEdit软件，基于注释和同源序列的局部BLAST挖掘候选关键基因。用实时定量聚合酶链反应分析基因的相对表达。此外，使用MEGA 6.0软件进行系统发育分析，挖掘CYP450基因。共产生156949个单基因，其中9021个在叶和根之间差异表达。首次获得了82个编码呋喃香豆素生物合成途径中8种酶的单基因。筛选了七个编码呋喃香豆素下游生物合成途径中关键酶的基因，这些基因在根中的表达量高于在叶中的表达量。26个候选CYP450单基因在根中大量表达，主要集中在CYP71、CYP85和CYP72家族。最后，我们筛选了102个差异表达转录因子（TFs）单基因。对该转录组进行了表征，这将有助于阐明该菌呋喃香豆素的生物合成途径，并为进一步研究呋喃香豆素提供宝贵的资源。

著录项

  • 来源
    《Journal of genetics》 |2020年第1期|共11页
  • 作者

    Song Jiejie; Luo Hongmei; Xu Zhichao; Zhang Yuxi; Xin Hua; Zhu Dan; Zhu Xunzhi; Liu Mengmeng; Wang Weiqing; Ren Hongwei; Chen Hongyu; Gao Ting;

  • 作者单位

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Chinese Acad Med Sci &

    Peking Union Med Coll Inst Med Plant Dev Minist Educ Key Lab Bioact Subst &

    Resources Utilizat Chinese Beijing 100193 Peoples R China;

    Chinese Acad Med Sci &

    Peking Union Med Coll Inst Med Plant Dev Minist Educ Key Lab Bioact Subst &

    Resources Utilizat Chinese Beijing 100193 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Inst Bot Nanjing 210014 Jiangsu Peoples R China;

    Jiangsu Univ Technol Sch Elect &

    Informat Engn Inst Bioinformat &

    Med Engn Changzhou 213001 Jiangsu Peoples R China;

    Shanghai Chenshan Bot Garden Shanghai Key Lab Plant Funct Genom &

    Resources Shanghai 201602 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

    Qingdao Agr Univ Coll Life Sci Key Lab Plant Biotechnol Univ Shandong Prov Qingdao 266109 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 遗传学;
  • 关键词

    transcriptome analysis; furanocoumarin; key genes; root-specific expression; Glehnia littoralis;

    机译：转录组分析;Furanocoumarin;关键基因;根特异性表达;Glehnia Littoralis;

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