...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >Analysis of genetic diversity in pigeon pea germplasm using retrotransposon-based molecular markers
【24h】

Analysis of genetic diversity in pigeon pea germplasm using retrotransposon-based molecular markers

机译：基于逆转脉冲分子标记的鸽豌豆种质遗传多样性分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pigeon pea (Cajanus cajan), an important legume crop is predominantly cultivated in tropical and subtropical regions of Asia and Africa. It is normally considered to have a low degree of genetic diversity, an impediment in undertaking crop improvement programmes. We have analysed genetic polymorphism of domesticated pigeon pea germplasm (47 accessions) across the world using earlier characterized panzee retrotransposon-based molecularmarkers. It was conjectured that since retrotransposons are interspersed throughout the genome, retroelements-based markers would be able to uncover polymorphism possibly inherent in the diversity of retroelement sequences. Two PCR-based techniques, sequence-specific amplified polymorphism (SSAP) and retrotransposon microsatellite amplified polymorphism (REMAP) were utilized for the analyses. We show that a considerable degree of polymorphism could be detected using these techniques. Three primer combinations in SSAP generated 297 amplified products across 47 accessions with an average of 99 amplicons per assay. Degree of polymorphism varied from 84-95%. In the REMAP assays, the number of amplicons was much less but up to 73% polymorphism could be detected. On the basis of similarity coefficients, dendrograms were constructed. The results demonstrate that the retrotransposon-based markers could serve as a better alternative for the assessment of genetic diversity in crops with apparent low genetic base.
机译：木豆是一种重要的豆类作物，主要种植在亚洲和非洲的热带和亚热带地区。人们通常认为它的遗传多样性程度较低，这是开展作物改良计划的一个障碍。我们使用基于panzee逆转录转座子的分子标记分析了世界各地驯化木豆种质（47份种质）的遗传多态性。据推测，由于反转录转座子散布在整个基因组中，基于反转录因子的标记将能够揭示反转录因子序列多样性中可能固有的多态性。两种基于PCR的技术，序列特异性扩增多态性（SSAP）和逆转录转座子微卫星扩增多态性（REMAP）被用于分析。我们表明，使用这些技术可以检测到相当程度的多态性。SSAP中的三个引物组合在47份材料中产生了297个扩增产物，每个分析平均99个扩增子。多态性程度从84%到95%不等。在REMAP分析中，扩增子的数量少得多，但可以检测到高达73%的多态性。在相似系数的基础上，构建了树状图。结果表明，基于反转录转座子的标记可以作为一种更好的选择，用于评估遗传基础明显较低的作物的遗传多样性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号