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首页> 外文期刊>Journal of genetics >QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers
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QTL identification for downy mildew resistance in cucumber using genetic linkage map based on SSR markers

机译：基于SSR标记的遗传联系地图，黄瓜中柔软霉菌抗性的QTL鉴定

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Fourteen cucumber lines were tested for genetic homozygosity and performed pairwise comparison to identify a pair with the highest DNA polymorphic level. Cucumber accessions CSL0067 and CSL0139 were selected to generate 315 F-2 populations. The genetic linkage map based on 66 polymorphic SSR markers was constructed. It composed of eight linkage groups (LGs) spanning 474.4 cM. Downy mildew disease reaction was evaluated in cotyledons, first and second true leaf on 7, 10, and 14 day after inoculation. The results showed that downy mildew resistance was controlled by multiple recessive genes. The susceptible to resistant ratio of F-2 progenies fit 9:7 susceptible/resistant segregation types corresponding to duplicate recessive epistasis. Fourteen QTLs were detected. The phenotypic variance ranged from 5.0 to 12.5%, while LOD values ranged from 3.538 to 9.165. Two major QTLs and two QTL hotspots were identified. Moreover, the additive effects data explained that these QTL reduced downy mildew susceptibility.
机译：对14个黄瓜品系进行遗传纯合性测试，并进行配对比较，以确定一对具有最高DNA多态性水平的黄瓜。选择黄瓜材料CSL0067和CSL0139产生315个F-2群体。构建了基于66个多态性SSR标记的遗传连锁图谱。它由八个连锁群（LGs）组成，跨度474.4厘米。在接种后第7、10和14天，在子叶、第一和第二真叶中评估霜霉病反应。结果表明，抗霜霉病性受多个隐性基因控制。F-2后代的感抗比符合9:7的感抗分离类型，对应于重复隐性上位性。共检测到14个qtl。表型变异范围为5.0-12.5%，LOD值范围为3.538-9.165。鉴定出两个主效QTL和两个QTL热点。此外，加性效应数据解释了这些QTL降低了霜霉病易感性。

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