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首页> 外文期刊>Journal of genetics >An integrative bioinformatics analysis of caprine GNAI3 gene
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An integrative bioinformatics analysis of caprine GNAI3 gene

机译：Caprine GNai3基因的一体化生物信息分析

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Goat is the most preferred domesticated animal in Indian subcontinent. However, the climatic change-induced heat stress causes a formidable challenge for maintaining optimum productivity. G protein subunit alpha i3 (GNAI3) is one of the genes that may have significant role in heat tolerance mechanism in goats. The caprine GNAI3 gene was searched for homology analysis and its three dimensional protein structure was predicted followed by its validation through in silico approach. Nucleotide sequence-based phylogenetic tree analysis showed that the caprine GNAI3 gene has close evolutionary relationship with that of Ovis aries. Homology modelling of caprine GNAI3 protein was done in MODELLER 9.18 (P1), PHYRE2 (P2), GENO3D (P3) and SWISS MODEL (P4). The modelled structures were further validated after observing the Ramachandran and hydrophobicity plots. In the best of three dimensional protein structure (P4 as produced by SWISS MODEL), 330 (98.8%), three (0.9%) and one (0.3%) amino acid residues were found in favoured region, allowed region and outlier region, respectively. Degree of hydrophobicity of the generated protein structures revealed the presence of alternate hydrophobic and hydrophilic regions. The ligand receptor interaction site of the predicted 3D model was traced out using Discovery Studio 3.5. STRING database revealed protein interactions with Plcb1, Plcb2, Plcb3 and other proteins of G family such as Gnb1, Gnb2, Gnb3,Gnb4, Gng2, Gng4 and Gpsm1. KEGG pathway maps revealed interaction with eNOS, iNOS, VEGF and MAPK, which are reported to be transcribed in response to heat stress. Thus, caprine GNAI3 can be used as a possible biomarker for studying heat tolerance mechanism in goats.
机译：山羊是印度次大陆最受欢迎的家养动物。然而，气候变化引起的热应激对维持最佳生产力造成了巨大挑战。G蛋白亚单位αi3（GNAI3）是山羊耐热机制中可能起重要作用的基因之一。寻找山羊GNAI3基因进行同源性分析，预测其三维蛋白质结构，然后通过电子方法进行验证。基于核苷酸序列的系统发育树分析表明，山羊GNAI3基因与绵羊GNAI3基因具有密切的进化关系。在MODELLER 9.18（P1）、PHYRE2（P2）、GENO3D（P3）和SWISS MODEL（P4）中对山羊GNAI3蛋白进行同源性建模。在观察Ramachandran图和疏水性图后，进一步验证了模型结构。在最佳三维蛋白质结构（瑞士模型产生的P4）中，在有利区、允许区和异常区分别发现330个（98.8%）、三个（0.9%）和一个（0.3%）氨基酸残基。生成的蛋白质结构的疏水性程度表明存在交替的疏水和亲水区域。使用Discovery Studio 3.5追踪预测的3D模型的配体-受体相互作用位点。STRING数据库揭示了蛋白质与Plcb1、Plcb2、Plcb3和其他G家族蛋白质的相互作用，如Gnb1、Gnb2、Gnb3、Gnb4、Gng2、Gng4和Gpsm1。KEGG通路图显示了与eNOS、iNOS、VEGF和MAPK的相互作用，据报道它们在热应激反应中被转录。因此，山羊GNAI3可以作为研究山羊耐热机制的一个可能的生物标记物。

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