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首页> 外文期刊>Journal of genetics >Identification of QTLs in oil palm (Elaeis guineensis Jacq.) using SSR markers through association mapping
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Identification of QTLs in oil palm (Elaeis guineensis Jacq.) using SSR markers through association mapping

机译：通过关联映射使用SSR标记的油棕（Elaeis Guineensis Jacq）的QTL鉴定

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Oil palm (Elaeis guineensis Jacq.) is a perennial vegetable and a high oil-yielding crop (4-6 t/ha). There is a large scope for increasing the oil yield by selecting elite planting material for breeding programme in germplasm evaluation, characterization and utilization. In the present study, a diverse range of 150 oil palm genotypes were characterized using 12 quantitative variables with 54 genomic microsatellite markers. A wide variation was observed in the morphological traits among indigenous populations. Highly significant and positive correlations were observed between vegetative dry matter (VDM) and total dry matter (TDM) (0.862), and height and height increment (0.838). The first two principal component analyses explained 67.7% of total variation among morphological traits. The genotypes IC0610001-59 (Pune-2) and IC0610001-60 (Pune-2) were found highly promising based on less height increment, more TDM with high yield. For the mapping study, general linear model (GLM) approach, quantitative-trait loci (QTL) for annual height increment, number of bunches, bunch yield and bunch index were linked to simple-sequence repeat (SSR) loci mEgCIR3649 with phenotypic variance of 15.08, 10.43, 11.74, 15.39. TDM and VDM were linked to mEgCIR0192 (27.34 and 24.19%), mEgCIR3684 (16.84 and 18.30%), SPSC00163 (18.8 and 15.39%) and mEgCIR0555 (16.47 and 18.81%), with at a significant threshold (P) level of <= 0.001 and by mixed linear model (MLM) approach. TDM was linked to mEgCIR0555 with phenotypic variance of 20.72%, bunch yield and bunch index were linked to mEgCIR2813 at phenotypic variance of 17.11% and 12.88%, respectively, at a significant threshold (P) level of <= 0.01.
机译：油棕是一种多年生蔬菜和高产油料作物（4-6吨/公顷）。在种质评价、性状鉴定和利用方面，选择优良的栽培材料进行育种，有很大的增产空间。在本研究中，使用12个数量变量和54个基因组微卫星标记，对150个不同的油棕基因型进行了表征。在土著群体中，观察到形态特征存在很大差异。营养体干物质（VDM）和总干物质（TDM）（0.862）以及高度和高度增量（0.838）之间呈极显著正相关。前两个主成分分析解释了形态性状总变异的67.7%。IC0610001-59（Pune-2）和IC0610001-60（Pune-2）基因型因高度增加少、TDM多、产量高而被认为是很有前途的基因型。在作图研究中，采用一般线性模型（GLM）方法，将年高增量、丛生数、丛生产量和丛生指数的数量性状位点（QTL）与简单序列重复（SSR）位点mEgCIR3649连锁，表型方差分别为15.08、10.43、11.74和15.39。TDM和VDM与mEgCIR0192（27.34%和24.19%）、mEgCIR3684（16.84%和18.30%）、SPSC00163（18.8%和15.39%）和mEgCIR0555（16.47%和18.81%）相关，显著阈值（P）水平<=0.001，并通过混合线性模型（MLM）方法。TDM与mEgCIR0555的表型方差为20.72%，而成束产量和成束指数与mEgCIR2813的表型方差分别为17.11%和12.88%，显著阈值（P）水平为<=0.01。

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