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首页> 外文期刊>Journal of insect biotechnology and sericology >Latest status of silkworm-associated microsporidians via pebrine inspection revealed by phylogenetic analyses
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Latest status of silkworm-associated microsporidians via pebrine inspection revealed by phylogenetic analyses

机译：通过系统发育分析揭示的卵虫检查的最新状态

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摘要

The microsporidium Nosema bombycis is a causal pathogen of pebrine disease in the silkworm, Bombyx mori. In the sericultural industry, pebrine inspection is intensively performed to eliminate microsporidia from B. mori to maintain strains and produceeggs commercially for various purposes. However, detailed properties of microsporidia isolated via pebrine inspection have not been mentioned. In this study, we analyzed microsporid-ian strains isolated from B. mori specimens during mass pebrine inspection by spore size comparison and phy-logenetic analysis of small subunit ribosomal RNA gene sequencing. Next, we elucidated the microsporidian fauna in the rearing room in five different years. A total of 310 samples were detected via pebrine inspection,47.4% of which were detected in the late autumn rearing season. Isolated microsporidian strains were classified into two groups based on spore size: Nosema or Vairimorpha and Pleistophora. Phylogenetic analysis suggested classification into four genera:Nosema, Vairimorpha, Cystosporogenes, and Endoreticulatus. Some strains classified into genera Nosema and Vairimorpha exhibited high genetic homology with N. bombycis and Vairimorpha sp. NIS-M11, which cause pebrine disease. Thus, conventional pebrine inspection facilitates the elimination of microsporidia, thereby preventing the spread of this disease. The genus Cystosporogenes was first detected from insects in Japan. However, classification by spore size is misunderstood in the genus Pleistophora, which infects B. mori larvae. Our present results suggest that the recent status of the microsporidian fauna in the silkworm rearing room is slightly different from that observed via conventional recognition of spore shapes under microscopy.
机译：家蚕微孢子虫是家蚕微粒子病的病原菌。在蚕业中，为了从桑蚕中消除微孢子虫，需要进行密集的微粒子检查，以维持菌株并为各种目的生产商业鸡蛋。然而，通过微粒子检测分离的微孢子虫的详细特性尚未提及。在这项研究中，我们通过孢子大小比较和小亚基核糖体RNA基因测序的物理遗传学分析，分析了在大规模检测期间从B.mori标本中分离的微孢子虫ian菌株。接下来，我们阐明了五年中饲养室内的微孢子虫区系。共检测到310份样品，其中47.4%是在深秋饲养季节检测到的。根据孢子大小将分离的微孢子虫菌株分为两类：微孢子虫或微孢子虫和更新世孢子虫。系统发育分析表明可分为四个属：微孢子虫属、瓦里莫巴属、囊孢子虫属和内鞭毛虫属。一些被归为微孢子虫属和瓦里莫巴属的菌株与引起佩林病的家蚕N.菌属和瓦里莫巴sp.NIS-M11具有高度的遗传同源性。因此，常规的微粒子检查有助于消除微孢子虫，从而防止这种疾病的传播。囊孢子虫属首次在日本的昆虫中发现。然而，在感染桑蚕幼虫的更新世虫属中，根据孢子大小进行分类是错误的。我们目前的结果表明，家蚕饲养室内微孢子虫区系的最新状况与通过显微镜下孢子形状的常规识别观察到的情况略有不同。

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