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首页> 外文期刊>Journal of mass spectrometry: JMS >Lasers for matrix-assisted laser desorption ionization
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Lasers for matrix-assisted laser desorption ionization

机译：用于矩阵辅助激光解吸电离的激光器

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摘要

Matrix-assisted laser desorption ionization (MALDI) was introduced 35 years ago and has advanced from a general method for producing intact ions from large biomolecules to wide use in applications ranging from bacteria identification to tissue imaging. MALDI was enabled by the development of high energy pulsed lasers that create ions from solid samples for analysis by mass spectrometry. The original lasers used for MALDI were ultraviolet fixed-wavelength nitrogen and Nd:YAG lasers, and a number of additional laser sources have been subsequently introduced with wavelengths ranging from the infrared to the ultraviolet and pulse widths from nanosecond to femtosecond. Wavelength tunable sources have been employed both in the IR and UV, and repetition rates have increased from tens of Hz to tens of kHz as MALDI has moved into mass spectrometry imaging. Dual-pulse configurations have been implemented with two lasers directed at the target or with a second laser creating ions in the plume of desorbed material. This review provides a brief history of the use of lasers for ionization in mass spectrometry and describes the various types of lasers and configurations used for MALDI.
机译：基质辅助激光解吸电离（MALDI）是35年前引入的，已经从一种从大生物分子中产生完整离子的通用方法发展到广泛应用，从细菌鉴定到组织成像。MALDI是由高能脉冲激光器的发展而实现的，该激光器从固体样品中产生离子，用于质谱分析。MALDI最初使用的激光器是紫外固定波长氮和Nd:YAG激光器，随后又引入了一些波长从红外到紫外，脉冲宽度从纳秒到飞秒的附加激光源。波长可调光源已在红外和紫外光谱中使用，随着MALDI进入质谱成像，重复频率已从几十Hz增加到几十kHz。双脉冲配置已经实现，两个激光指向目标，或者第二个激光在解吸物质的羽流中产生离子。本综述简要介绍了激光用于质谱电离的历史，并描述了用于MALDI的各种类型的激光和配置。

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