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首页> 外文期刊>Journal of Medical Entomology >Identification and Characterization of Aminopeptidase N 1 Gene of the Indian Malaria Vector Anopheles culicifacies (Diptera: Culicidae)
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Identification and Characterization of Aminopeptidase N 1 Gene of the Indian Malaria Vector Anopheles culicifacies (Diptera: Culicidae)

机译：印度疟原虫醋酸纤维素醋酸氨基肽酶N 1基因的鉴定与表征（Diptera：Culicidae）

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摘要

Aminopeptidase N1 (APN) is one of the important enzymes involved in blood digestion and is up-regulated along with several other enzymes in response to bloodmeal ingestion. APN is a zinc metalloprotease that cleaves one amino acid residue at a time from the amino terminus of the protein. The APN1 gene of the Indian malaria vector Anopheles culicifacies Giles was cloned and characterized. The An. culicifacies APN1 (AcAPN1) gene has an Open Reading Frame of 3084 basepairs which encodes a putative protein of 1027 amino acids. The coding region of the gene shares 81% and 78% similarity to the APN1 genes found in An. stephensi (Diptera: Culicidae) and An. gambiae (Diptera: Culicidae), respectively. The organization of the APN1 gene was studied in available mosquito genomes and a three-dimensional structure of AcAPN1 modeled using homology structure modeling. The enzymatic active site was predicted to consist of HEYAH and GAMEN amino acid residues, and a comparison of the protein sequences among different genera revealed the conservation of zinc-binding residues. The expression pattern of AcAPN1 showed that the gene was expressed rapidly in response to the ingestion of the bloodmeal and therefore this gene may be used to exploit its promoter region as an antiparasite candidate molecule.
机译：氨肽酶N1（APN）是参与血液消化的重要酶之一，与其他几种酶一起在血粉摄入时上调。APN是一种锌金属蛋白酶，一次从蛋白质的氨基末端切割一个氨基酸残基。克隆并鉴定了印度疟疾媒介库蚊的APN1基因。这是一个很好的例子。库蚊APN1（AcAPN1）基因具有3084个碱基对的开放阅读框，编码1027个氨基酸的蛋白质。该基因的编码区与人类基因组中发现的APN1基因有81%和78%的相似性。stephensi（双翅目：库蚊科）和。冈比亚（双翅目：库蚊科）。在可用的蚊子基因组中研究了APN1基因的组织，并使用同源结构建模对AcAPN1的三维结构进行了建模。酶活性位点由HEYAH和GAMEN氨基酸残基组成，不同属间蛋白质序列的比较揭示了锌结合残基的保守性。AcAPN1的表达模式表明，该基因在摄入血粉后迅速表达，因此该基因可用于开发其启动子区域作为抗寄生虫候选分子。

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