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首页> 外文期刊>Journal of Medical Entomology >Role of Chrysomya albiceps (Diptera: Calliphoridae) and Musca domestica (Diptera: Muscidae) Maggot Crop Contents in Identifying Unknown Cadavers
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Role of Chrysomya albiceps (Diptera: Calliphoridae) and Musca domestica (Diptera: Muscidae) Maggot Crop Contents in Identifying Unknown Cadavers

机译：Chrysomya albicsps（Diptera：Calliphoridae）和Musca domestica（Diptera：Muscidae）Maggot作物内容在确定未知的尸体时的作用

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Forensic entomology focuses on the analysis of insect larvae present at crime scenes to help identify unknown cadavers. Carrion-feeding maggots store food in a crop located at the anterior end of the gut. DNA recovered from the crop can be amplified, sequenced, and identified to determine the origin of the food. This information could help investigators to identify a missing victim if maggots are discovered at a crime scene in the absence of a corpse. Chrysomya albiceps (Wiedemann) and Musca domestica (Linnaeus) are primary forensic species. Little or no information on the identification of unknown cadavers using C. albiceps and M. domestica larvae is available, and we aimed to compare the effectiveness of using the crop contents of instars of C. albiceps and M. domestica larvae to identify corpses at different time intervals. Two hundred and forty larvae of both species were reared on rat and/or mouse liver, and DNA from crop contents was extracted after different time intervals from different instar larvae. DNA was amplified using specific primers that match the mitochondrial cytochrome oxidase subunit I (mt COI) gene of the food source. Results showed that food provided to larvae affected life span and the ability to be used to identify unknown cadavers. Chrysomya albiceps larvae proved more useful than M. domestica larvae. Moreover, crop contents of third instar larvae of both species fed for 24 h are more useful than contents from other time intervals in identifying unknown tissues. Results are promising and may help investigators to identify unknown/missing victims.
机译：法医昆虫学侧重于分析犯罪现场的昆虫幼虫，以帮助识别未知尸体。食腐蛆将食物储存在位于肠道前端的作物中。从作物中提取的DNA可以进行扩增、测序和鉴定，以确定食物的来源。如果在没有尸体的情况下在犯罪现场发现蛆虫，这些信息可以帮助调查人员确定失踪的受害者。白花金蝇（Wiedemann）和家蝇（Linnaeus）是主要的法医学物种。关于使用白僵菌和家蝇幼虫识别未知尸体的信息很少或没有，我们的目的是比较在不同时间间隔使用白僵菌和家蝇幼虫龄期的作物含量来识别尸体的有效性。在大鼠和/或小鼠肝脏上饲养这两个物种的240只幼虫，并在不同时间间隔后从不同龄期的幼虫中提取作物内容物中的DNA。使用与食物来源的线粒体细胞色素氧化酶亚单位I（mt COI）基因匹配的特异性引物扩增DNA。结果表明，提供给幼虫的食物会影响其寿命和用于识别未知尸体的能力。白化金蝇幼虫被证明比家蝇幼虫更有用。此外，在识别未知组织方面，两个物种的三龄幼虫饲喂24小时后的作物含量比其他时间间隔的含量更有用。结果是有希望的，可能有助于调查人员确定未知/失踪的受害者。

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