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首页> 外文期刊>Journal of neuroendocrinology >Transcriptional regulation of prolactin in a euryhaline teleost: Characterisation of gene promoters through in silico and transcriptome analyses
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Transcriptional regulation of prolactin in a euryhaline teleost: Characterisation of gene promoters through in silico and transcriptome analyses

机译：Euryhaline Teactost中催乳素的转录调节：在硅和转录组分析中通过基因启动子的表征

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摘要

The sensitivity of prolactin (Prl) cells of the Mozambique tilapia (Oreochromis mos-sambicus) pituitary to variations in extracellular osmolality enables investigations into how osmoreception underlies patterns of hormone secretion. Through the actions of their main secretory products, Prl cells play a key role in supporting hydromineral balance of fishes by controlling the major osmoregulatory organs (ie, gill, intestine and kidney). The release of Prl from isolated cells of the rostral pars distalis (RPD) occurs in direct response to physiologically relevant reductions in extracellular osmolality. Although the particular signal transduction pathways that link osmotic conditions to Prl secretion have been identified, the processes that underlie hyposmotic induction of prl gene expression remain unknown. In this short review, we describe two distinct tilapia gene loci that encode Prl177 and Prl188. From our in silico analyses of prl177 and prl188 promoter regions (approximately 1000 bp) and a transcriptome analysis of RPDs from fresh water (FW)- and seawater (SW)-acclimated tilapia, we propose a working model for how multiple transcription factors link osmoreceptive processes with adaptive patterns of prl177 and prl188 gene expression. We confirmed via RNA-sequencing and a quantitative polymerase chain reaction that multiple transcription factors emerging as predicted regulators of prl gene expression are expressed in the RPD of tilapia. In particular, gene transcripts encoding pou1f stat3, creb3l pbxip1a and stat1a were highly expressed; creb3l pbxip1a and stat1a were elevated in fish acclimated to SW vs FW. Combined, our in silico and transcriptome analyses set a path for resolving how adaptive patterns of Prl secretion are achieved via the integration of osmoreceptive processes with the control of prl gene transcription.
机译：莫桑比克罗非鱼（Oreochromis mos sambicus）垂体催乳素（Prl）细胞对细胞外渗透压变化的敏感性使我们能够研究渗透压感受是如何构成激素分泌模式的。通过其主要分泌产物的作用，Prl细胞通过控制主要渗透调节器官（即鳃、肠和肾）在支持鱼类水矿物质平衡方面发挥关键作用。从嘴侧远侧部（RPD）的分离细胞释放Prl是对细胞外渗透压生理相关降低的直接反应。虽然已经确定了将渗透条件与Prl分泌联系在一起的特定信号转导途径，但低渗诱导Prl基因表达的过程仍然未知。在这篇简短的综述中，我们描述了编码Prl177和Prl188的两个不同的罗非鱼基因座。根据我们对prl177和prl188启动子区域（约1000 bp）的电子分析，以及对淡水（FW）和海水（SW）驯化罗非鱼RPD的转录组分析，我们提出了一个工作模型，用于研究多种转录因子如何将渗透感受过程与prl177和prl188基因表达的适应性模式联系起来。我们通过RNA测序和定量聚合酶链反应证实，罗非鱼的RPD中表达了多种转录因子，这些转录因子是prl基因表达的预测调节因子。尤其是编码pou1f stat3、creb3l pbxip1a和stat1a的基因转录本得到了高度表达；在适应SW和FW的鱼类中，creb3l pbxip1a和stat1a升高。我们的电子和转录组分析结合起来，为解决如何通过渗透感受过程与Prl基因转录控制的整合来实现Prl分泌的适应性模式提供了一条途径。

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