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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Preparation of nano cationic liposome as carrier membrane for polyhexamethylene biguanide chloride through various methods utilizing higher antibacterial activities with low cell toxicity
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Preparation of nano cationic liposome as carrier membrane for polyhexamethylene biguanide chloride through various methods utilizing higher antibacterial activities with low cell toxicity

机译：用较高抗菌活性的各种方法制备纳米阳离子脂质体作为多烷亚甲基双甲基双氯化物的载体膜

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摘要

This study suggested successful encapsulation of polyhexamethylene biguanide chloride (PHMB) into nano cationic liposome as a biocompatible antibacterial agent with less cytotoxicity and higher activities. Phosphatidylcholine, cholesterol and stearylamine were used to prepare nano cationic liposome using thin film hydration method along with sonication or homogeniser. Sonication was more effective in PHMB loaded nano cationic liposome preparation with smaller size (34 nm). FTIR, H-1 NMR and XRD analyses were used to confirm the encapsulation of PHMB into nano cationic liposome. PHMB inclusion in nano cationic liposome was beneficial for increased antibacterial activity against Staphylococcus aureus and Escherichia coli. PHMB-loaded cationic liposome enables to deliver high concentrations of the antibacterial agent into the infectious cell. The cytotoxicity of PHMB entrapped in positively charged liposome was prominently reduced showing no significant visible detrimental effect on normal primary human skin fibroblast cell lines morphology confirming the effective role of cationic liposome encapsulation. Comparing with PHMB alone, encapsulation of PHMB in nano cationic liposome resulted in significant increase in cell viability from 2.4 to 63%.
机译：本研究表明，聚六亚甲基双胍氯化物（PHMB）作为一种生物相容性抗菌剂成功地包封到纳米阳离子脂质体中，具有较小的细胞毒性和较高的活性。以磷脂酰胆碱、胆固醇和硬脂胺为原料，采用薄膜水化法制备纳米阳离子脂质体，并辅以超声或均化剂。超声处理在粒径较小（34nm）的PHMB纳米阳离子脂质体制备中更有效。FTIR、H-1 NMR和XRD分析证实PHMB被包裹在纳米阳离子脂质体中。纳米阳离子脂质体中的PHMB包合物有利于提高对金黄色葡萄球菌和大肠杆菌的抗菌活性。PHMB负载的阳离子脂质体能够将高浓度的抗菌剂输送到感染细胞中。包埋在带正电脂质体中的PHMB的细胞毒性显著降低，对正常的原代人皮肤成纤维细胞系形态没有明显的有害影响，证实了阳离子脂质体包埋的有效作用。与单独使用PHMB相比，将PHMB包埋在纳米阳离子脂质体中可使细胞活力从2.4%显著提高到63%。

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