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首页> 外文期刊>Journal of Microencapsulation: Microcapsules Liposomes Nanoparticles Microcells Microspheres >Preparation, characterisation and cell viability of encapsulated Trichoderma asperellum in alginate beads
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Preparation, characterisation and cell viability of encapsulated Trichoderma asperellum in alginate beads

机译：在海藻酸盐珠粒中封装的Trichoderma asperellum的制备，表征和细胞活力

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Aim: The encapsulation of Trichoderma asperellum BRM-29104 using Ca-alginate matrix was evaluated. Methods: Intact and freeze-dried beads containing submerged conidia and microsclerotia (MS) of T. asperellum grown in liquid culture were prepared and characterised. Beads were stored at 8, 25, and 35 degrees C over 120 days. Results: The mean sizes of beads before and after freeze-drying were 2.5 +/- 0.2 mm and 1.5 x 1.1 mm (+/- 0.1 mm), respectively. Freeze-dried beads stored at 8 degrees C were more effective in maintaining conidia concentration, while MS concentrations yielded 10(2) MS/g for both beads at 8 and 25 degrees C. The concentration of viable cells in freeze-dried beads stored at 8 degrees C attained 3.0 x 10(8) CFU/g after 120 days. FIRT analysis showed an interaction between the alginate and the cell wall of the fungus. Conclusion: These findings support the use of alginate beads followed by freeze drying and cold storage for maintenance of viability of T. asperellum.
机译：目的：评价海藻酸钙基质对木霉BRM-29104的包封作用。方法：制备完整和冷冻干燥的小球，其中含有液体培养中生长的山杨木的浸没分生孢子和微透明体（MS），并对其进行表征。珠子在8摄氏度、25摄氏度和35摄氏度下储存120天。结果：冷冻干燥前后的平均粒径分别为2.5+/-0.2mm和1.5x1.1mm（+/-0.1mm）。在8摄氏度下储存的冷冻干燥珠粒在维持分生孢子浓度方面更有效，而在8摄氏度和25摄氏度下，MS浓度为10（2）MS/g。在8摄氏度下储存的冷冻干燥珠粒中的活细胞浓度在120天后达到3.0 x 10（8）CFU/g。FIRT分析显示褐藻酸盐和真菌细胞壁之间存在相互作用。结论：这些研究结果支持使用海藻酸钠珠，然后冷冻干燥和冷藏，以维持山杨木霉的生存能力。

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