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首页> 外文期刊>Journal of oral rehabilitation >Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis
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Immunohistochemical panel of degenerated articular discs from patients with temporomandibular joint osteoarthritis

机译：颞下颌关节骨关节炎患者退化关节椎间盘的免疫组织化学板

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Background Temporomandibular joint osteoarthritis (TMJOA) is a progressive degenerative disease caused by imbalance between anabolic and catabolic stimuli. Objective The aim of this study was to evaluate histopathological changes, collagen degeneration and the expression of eleven TMJOA biomarkers in articular discs. Methods Specimens were obtained from eight female patients submitted to discectomy. Discs were divided into anterior band (AB), intermediate zone (IZ) and posterior band (PB) for computerised histomorphometric analyses. Each was assigned a histopathological degeneration score (HDS). Collagen degeneration was assessed with Picrosirius-polarisation method. Biomarkers were evaluated through immunohistochemistry, including IGF-1, OPG, VEGF, TNF-alpha, FGF-23, IHH, MMP-3, MMP-9, TGF-beta(1), BMP-2 and WNT-3. Image processing software was used to calculate average immature collagen ratios and immunostained areas. Spearman rank tests were applied to verify correlations, with significance level of 0.05. Results The HDS showed negative correlation with expression of VEGF in IZ and PB (P < .05) and positive with TNF-alpha in AB (P < .01). Collagen degeneration correlated with TGF-beta(1)(P < .05), BMP-2 (P < .01) and IHH (P < .05) immunostained areas in the IZ; TGF-beta 1,BMP-2 and IHH expression correlated among each other in AB and IZ (P < .05). Conclusion Angiogenesis and tissue fragmentation may result from aberrant physiologic responses mediated by VEGF and TNF-alpha, compromising TMJ discs during OA progression. The expression of TGF-beta 1,BMP-2 and IHH could be related to collagen degeneration in displaced discs and may participate in TMJOA pathogenesis.
机译：背景颞下颌关节骨关节炎（TMJOA）是一种由合成代谢和分解代谢刺激失衡引起的进行性退行性疾病。目的研究关节盘组织病理学改变、胶原变性及11种TMJOA生物标志物的表达。方法从8例接受椎间盘切除术的女性患者中获取标本。将椎间盘分为前带（AB）、中间带（IZ）和后带（PB），进行计算机组织形态计量学分析。每个人都被分配了组织病理学退行性变评分（HDS）。胶原变性采用Picrosirius偏振法进行评估。通过免疫组化评估生物标志物，包括IGF-1、OPG、VEGF、TNF-α、FGF-23、IHH、MMP-3、MMP-9、TGF-β（1）、BMP-2和WNT-3。使用图像处理软件计算平均未成熟胶原比率和免疫染色面积。Spearman秩检验用于验证相关性，显著性水平为0.05。结果HDS与IZ和PB中VEGF的表达呈负相关（P<0.05），与AB中TNF-α的表达呈阳性（P<0.01）。胶原变性与IZ中TGF-β（1）（P<0.05）、BMP-2（P<0.01）和IHH（P<0.05）免疫染色区域相关；在AB和IZ中，TGFβ1、BMP-2和IHH的表达相互关联（P<0.05）。结论血管生成和组织碎裂可能是由VEGF和TNFα介导的异常生理反应引起的，在OA进展过程中损害了TMJ椎间盘。TGFβ1、BMP-2和IHH的表达可能与移位椎间盘的胶原变性有关，并可能参与TMJOA的发病机制。

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