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首页> 外文期刊>Journal of Reproductive Immunology >Uterine CD11c+cells induce the development of paternal antigen-specific Tregs via seminal plasma priming
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Uterine CD11c+cells induce the development of paternal antigen-specific Tregs via seminal plasma priming

机译：子宫CD11c +细胞通过精液灌注诱导父抗原特异性Tregs的发育

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摘要

Tolerogenic dendritic cells (tDCs) play a central role in the development of paternal antigen-specific regulatory T cells (Tregs) during pregnancy. We examined whether uterine CD11c(+) antigen presenting cells (APC) induced paternal antigen-specific tolerance in allogeneic pregnant mice. Female BALB/c mice were mated with male DBA/2 mice, and their surface markers of APCs were studied using flow cytometry. After allogeneic mating, the uterine APCs exhibited significantly decreased expression of major histocompatibility complex (MHC) class II on day 3.5 post-coitus (pc) and day 5.5 pc. To analyze how seminal fluid affects surface markers of APCs, female BALB/c mice were mated with male mice that had undergone seminal vesicle excision (SVX). No reductions of MHC class II expression on APCs were seen in these mice. To analyze APC functions, a mixed lymphoid reaction (MLR) assay to paternal splenocytes was performed. Uterine APCs from allogeneic pregnant mice significantly suppressed the MLR reaction, but APCs from SVX mated mice did not suppress the MLR reaction Uterine APCs induced paternal antigen (Mls1a)-specific Treg development in vitro, but not in mice that mated with allogeneic SVX mice. These findings suggest that seminal fluid priming expands the paternal antigen-specific Treg population by inducing APCs development.
机译：耐受性树突状细胞（TDC）在妊娠期父系抗原特异性调节性T细胞（Treg）的发育中起着核心作用。我们检测了子宫CD11c（+）抗原呈递细胞（APC）是否诱导异基因妊娠小鼠的父系抗原特异性耐受。雌性BALB/c小鼠与雄性DBA/2小鼠交配，并使用流式细胞术研究其APC表面标记物。在异基因交配后，在性交后3.5天和性交后5.5天，子宫APC的主要组织相容性复合体（MHC）II类表达显著降低。为了分析精液如何影响APC的表面标记物，将雌性BALB/c小鼠与接受精囊切除（SVX）的雄性小鼠交配。在这些小鼠中，未观察到APC上MHC II类表达的减少。为了分析APC的功能，对父系脾细胞进行了混合淋巴细胞反应（MLR）分析。来自异基因怀孕小鼠的子宫APC显著抑制MLR反应，但来自SVX交配小鼠的APC在体外不抑制MLR反应-子宫APC诱导的父系抗原（Mls1a）-特异性Treg发育，但在与异基因SVX小鼠交配的小鼠中不抑制。这些发现表明，精液启动通过诱导APC的发育，扩大了父系抗原特异性Treg群体。

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