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首页> 外文期刊>Journal of Reproductive Immunology >Inflammation related to high-mobility group box-1 in endometrial ovarian cyst
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Inflammation related to high-mobility group box-1 in endometrial ovarian cyst

机译：与子宫内膜卵巢囊肿中高迁移率组箱-1相关的炎症

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摘要

Endometriosis is a chronic inflammatory disease often associated with dysmenorrhea, infertility, adenomyosis, and endometrial ovarian cyst (EOC). In particular, EOC can sometimes become malignant in a longitudinal follow-up. This study aimed to investigate the involvement of high-mobility group box-1 (HMGB1) in an inflammatory milieu and the characteristics of immune cells in EOC. The samples were obtained from patients who underwent ovarian cystectomy for benign ovarian cyst. The participants were divided into two groups: patients with EOC (EOC group) and those without EOC (nEOC group). We divided a part of the removed ovary into small sections and isolated the tissue cells. Thereafter, the cytoplasmic HMGB1 levels in DCs, macrophages, and non-(i)mmune cells were analyzed by flow cytometry. We also evaluated the proportions of immune, T, NK, iNKT, NK, and regulatory T (Treg) cells. Results showed that the DCs, macrophages, and non-immune cells of EOC had significantly higher cytoplasmic HMGB1 levels than those of nEOC. The expression of CD69 and CD107a on CD8(+) T and CD4(+) T cells of EOC was also more enhanced than that of nEOC. Furthermore, the M2 macrophages and Tregs highly accumulated in EOC. These results indicate that HMGB1 may aggravate chronic inflammation related to T-cell activation and simultaneously facilitate development of the immunosuppressive milieu in EOCs.
机译：子宫内膜异位症是一种慢性炎症性疾病，常与痛经、不孕、子宫腺肌病和子宫内膜卵巢囊肿（EOC）有关。特别是，在纵向随访中，EOC有时会变得恶性。本研究旨在探讨高迁移率族蛋白B1（HMGB1）在炎症环境中的作用以及EOC中免疫细胞的特征。样本取自因良性卵巢囊肿行卵巢囊肿切除术的患者。参与者被分为两组：EOC患者（EOC组）和非EOC患者（nEOC组）。我们将部分切除的卵巢分割成小部分，并分离组织细胞。此后，通过流式细胞术分析DC、巨噬细胞和非（i）免疫细胞中的细胞质HMGB1水平。我们还评估了免疫、T、NK、iNKT、NK和调节性T（Treg）细胞的比例。结果表明，EOC的DC、巨噬细胞和非免疫细胞的细胞质HMGB1水平显著高于nEOC。EOC的CD8（+）T细胞和CD4（+）T细胞上CD69和CD107a的表达也比nEOC的表达增强。此外，M2巨噬细胞和Treg在EOC中高度积累。这些结果表明HMGB1可能会加重与T细胞活化相关的慢性炎症，同时促进EOC中免疫抑制环境的发展。

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