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首页> 外文期刊>Journal of Parasitology >ACROEIMERIA LINERI (MCALLISTER, UPTON, FREED, 1988) PAPERNA AND LANDSBERG, 1989 IN MEDITERRANEAN GECKOS (HEMIDACTYLUS TURCICUS): OOCYST MORPHOMETRICS, ENDOGENOUS DEVELOPMENTAL STAGES, AND MOLECULAR SEQUENCING SUPPORT ITS PLACEMENT INTO ACROEIMERIA
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ACROEIMERIA LINERI (MCALLISTER, UPTON, FREED, 1988) PAPERNA AND LANDSBERG, 1989 IN MEDITERRANEAN GECKOS (HEMIDACTYLUS TURCICUS): OOCYST MORPHOMETRICS, ENDOGENOUS DEVELOPMENTAL STAGES, AND MOLECULAR SEQUENCING SUPPORT ITS PLACEMENT INTO ACROEIMERIA

机译：Acroeimeria Lineri（Mcallister，Upton，Freed，1988）Paperna和Landsberg，1989年在地中海壁虎（Hemidactylus Turcicus）：卵囊的形态识别性，内源性发育阶段和分子测序支持其放入炭疽物质

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Between June 2016 and June 2019, we surveyed 62 Mediterranean geckos, Hemidactylus turcicus, from Abu Rawash, Giza, Egypt, for the presence of endoparasites. In June 2016, we found 3 individuals to be infected with Eimeria lineri. We studied the morphology and inner structures of its sporulated oocysts, and the locations of its intestinal endogenous stages. We also extracted genomic DNA from these sporulated oocysts and successfully sequenced a 632-bp fragment of the 18S rRNA gene. Phylogenetic analyses using this partial sequence allowed us to support previous studies that assigned E. lineri to the genus Acroeimeria. Our consensus sequence was used to query similar 18S rDNA sequences from GenBank, and 14 sequences were selected. The phylogenetic analysis inferred by maximum likelihood and Bayesian inference methods gave similar results, as both separated the sequences into 2 clades: (1) a monophyletic group of Goussia species (from fish); and (2) a strongly supported clade that separated 4 Choleoeimeria species from a polyphyletic group of species that clustered A. lineri with 3 other Acroeimeria species and 3 Eimeria species from lizards, including Eimeria tiliquae from Tiliqua rugosa (Gray, 1825), Eimeria tokayae from Gecko gecko (L., 1758), and Eimeria eutropidis from Eutropis macularia (Blyth, 1853). Our study supports the placement of E. lineri into the Acroeimeria and contributes additional life history information toward understanding the evolutionary origin of the Eimeria-like species that have sporocysts without Stieda bodies in their oocysts and that infect saurian reptiles. We also support the concept that several traits (morphological, endogenous, and gene sequences) are both necessary and important for authors to include when making generic reassignments within the eimeriid coccidia.
机译：2016年6月至2019年6月期间，我们调查了埃及吉萨阿布·拉瓦什的62只地中海壁虎（Hemidactylus turcicus）是否存在内寄生虫。2016年6月，我们发现3人感染莱茵球虫。我们研究了其孢子化卵囊的形态和内部结构，以及其肠道内源性阶段的位置。我们还从这些孢子化卵囊中提取了基因组DNA，并成功测序了18S rRNA基因的632 bp片段。利用这一部分序列进行的系统发育分析使我们能够支持以前的研究，即将E.lineri归入顶球虫属。我们的一致序列用于从GenBank查询相似的18S rDNA序列，并选择了14个序列。通过最大似然和贝叶斯推断方法推断的系统发育分析给出了类似的结果，因为这两种方法都将序列分为两个分支：（1）Goussia物种的单系群（来自鱼类）；（2）一个强有力的支持分支，将4个胆源性伊美耳球虫物种从一个多系物种群中分离出来，该物种群将a.lineri与其他3个顶球虫物种和3个蜥蜴艾美耳球虫物种聚集在一起，包括Tiliqua rugosa的tiliquae艾美耳球虫（Gray，1825），Gecko Gecko的tokayae艾美耳球虫（L.，1758），以及Eutropis Eutropis macularia（Blyth，1853）。我们的研究支持E.lineri进入顶球虫，并为了解类似艾美球虫的物种的进化起源提供了额外的生活史信息，这些物种的卵囊中有孢子囊，但没有Stieda小体，感染蜥蜴爬行动物。我们还支持这样一个概念，即作者在对艾美球虫进行基因重组时，需要考虑的几个特征（形态、内源性和基因序列）既必要又重要。

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