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首页> 外文期刊>Journal of Parasitology >ADDITIONAL MORPHOLOGICAL FEATURES AND MOLECULAR DATA OF PARACAMALLANUS CYATHOPHARYNX (NEMATODA: CAMALLANIDAE) INFECTING CLARIAS GARIEPINUS (ACTINOPTERYGII: CLARIIDAE) IN KENYA
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ADDITIONAL MORPHOLOGICAL FEATURES AND MOLECULAR DATA OF PARACAMALLANUS CYATHOPHARYNX (NEMATODA: CAMALLANIDAE) INFECTING CLARIAS GARIEPINUS (ACTINOPTERYGII: CLARIIDAE) IN KENYA

机译:Paracamalanalanus性腺ant(墨西哥州:Camallanidae)的另外的形态学特征和分子数据感染克拿大克拉斯加里彭(Actinopterygii:Clariidae)

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摘要

This study provides additional taxonomic features based on scanning electron microscopy (SEM) and molecular data for Paracamallanus cyathopharynx (Baylis, 1923) (Nematoda: Camallanidae). Parasites were collected from the posterior end of the intestine of cultured freshwater Sharptooth catfish Clarias gariepinus (Burchell, 1822) from Kibos fish farm, Kisumu County, Kenya, from Kibos December 2017 to March 2018. Additional taxonomic features recorded for P. cyathopharynx include the occurrence of 4-5 equal length digitate processes on the caudal end of the female, 4 Kenya processes (2 smaller and 2 larger) on the male caudal end, and a description of the shape of the distal tip of the right spicule. The study provides SEM images of the exposed sclerotized buccal capsule. This gives more information on the tridents, the sclerotized plate that extends laterally from the buccal capsule, and the narrow isthmus separating the anterior buccal capsule from the posterior. The prevalence, intensity, mean intensity, and mean abundance was 52.91%, 2-38, 12.37 and 6.54, respectively. 18S rDNA fragments were amplified, sequenced, and compared to other camallanid taxa, and 18S data confirmed the identity of the newly obtained sequences (MN396556) as that of P. cyathopharynx, being identical to sequence DQ813445 from Tanzania. This represents the first geographical record of P. cyathopharynx in Kenya.
机译:本研究基于扫描电子显微镜(SEM)和分子数据提供了cyathopharynx副卡氏线虫(Baylis,1923)(线虫纲:卡氏线虫科)的额外分类特征。2017年12月至2018年3月,从肯尼亚基苏木县基博斯养鱼场养殖的淡水锐齿鲶鱼Clarias gariepinus(Burchell,1822)的肠道后端收集寄生虫。Cyathopharyx的其他分类学特征包括在雌性尾端出现4-5个等长指突,在雄性尾端出现4个肯尼亚突(2个较小,2个较大),以及对右骨针远端形状的描述。该研究提供了暴露的硬化口腔胶囊的SEM图像。这提供了更多关于三叉齿、从颊囊横向延伸的硬化板以及将前颊囊与后颊囊分离的狭窄峡部的信息。患病率、强度、平均强度和平均丰度分别为52.91%、2-38%、12.37和6.54。对18S rDNA片段进行扩增、测序,并与其他camallanid分类群进行比较,18S数据证实了新获得的序列(MN396556)与cyathopharynx的序列相同,与坦桑尼亚的序列DQ813445相同。这代表了肯尼亚第一个关于猪牙鲆的地理记录。

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