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首页> 外文期刊>Journal of stroke and cerebrovascular diseases: The official journal of National Stroke Association >Combination of Scalp Acupuncture with Exercise Therapy Effectively Counteracts Ischemic Brain Injury in Rats
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Combination of Scalp Acupuncture with Exercise Therapy Effectively Counteracts Ischemic Brain Injury in Rats

机译：头皮针灸与运动疗法的组合有效地抵消了大鼠缺血性脑损伤

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Background: Stroke is one of the leading causes of death and disability worldwide. Scalp acupuncture and exercise therapy have been proven as two effective methods for the treatment of stroke. However, their combined action and mechanisms have not been fully elucidated. The present study aimed to investigate the protective effect of scalp acupuncture combined with exercise therapy on neurons in rats with ischemic brain injury. Methods: 100 rats were randomly divided into 5 groups including sham group, model group, acupuncture group, rehabilitation group, and experimental group (scalp acupuncture combined with exercise therapy). Middle cerebral artery occlusion (MCAO) model in rats was established according to Longa modified suture method to mimic ischemic stroke. The modified Bedexer's neurological function score was used to evaluate the neurological deficits of rats and the brain infarct volume was measured using 2, 3, 5-triphenyl tetrazolium chloride monohydrate (TTC) staining. Moreover, the apoptosis in the hippocampus was detected by western blotting and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The pro-inflammatory cytokines such as interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha), reactive oxygen species (ROS) and superoxide dismutase (SOD) were determined by corresponding kits. Immunohistochemistry or immunofluorescence was performed to detect the expression of brain-derived neurotrophic factor (BDNF), S100 beta and glial fibrillary acidic protein (GFAP) in the hippocampi of rats. Results: The neurological deficit score, the expression levels of apoptotic factors such as cleaved caspase-3 and Bax, and the TUNEL-positive cell rate of the experimental group were significantly lower than those of the acupuncture group and the rehabilitation group. However, apoptosis inhibitor Bcl-2 showed downregulated expression in the MCAO model rats but this trend was reverted by single and combinatorial treatments. In addition, the contents of TNF-alpha, IL-1 beta and ROS in the acupuncture group and the rehabilitation group were significantly lower than those in the model group, but higher than the experimental group. While the opposite results were obtained in SOD activity. Furthermore, compared with the model group, the ratios of BDNF, S100 beta, and GFAP-positive cells in the acupuncture, rehabilitation and experimental groups were significantly increased, and the highest ratios were recorded in the experimental group. Conclusions: This study demonstrated that scalp acupuncture combined with exercise therapy effectively counteracts ischemic brain injury via the downregulation of pro-inflammatory mediators and ROS, the increased production of the antioxidant enzyme SOD, neurotrophic factor BDNF and astrocyte activities.
机译：背景：中风是世界范围内导致死亡和残疾的主要原因之一。头皮针和运动疗法已被证明是治疗中风的两种有效方法。然而，它们的联合作用和机制尚未完全阐明。本研究旨在探讨头针结合运动疗法对缺血性脑损伤大鼠神经元的保护作用。方法：将100只大鼠随机分为假手术组、模型组、针刺组、康复组和实验组（头针结合运动疗法）。采用Longa改良缝合法建立大鼠大脑中动脉闭塞（MCAO）模型，模拟缺血性脑卒中。采用改良的Bedexer神经功能评分法评估大鼠的神经功能缺损，并采用2,3,5-三苯基四氮唑氯化物一水合物（TTC）染色法测量脑梗塞体积。此外，通过western印迹和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）检测海马细胞凋亡。用相应试剂盒检测促炎细胞因子白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNFα）、活性氧（ROS）和超氧化物歧化酶（SOD）。采用免疫组织化学或免疫荧光法检测大鼠海马脑源性神经营养因子（BDNF）、S100β和胶质纤维酸性蛋白（GFAP）的表达。结果：实验组神经功能缺损评分、凋亡因子（如切割的caspase-3和Bax）的表达水平以及TUNEL阳性细胞率均显著低于针刺组和康复组。然而，在MCAO模型大鼠中，凋亡抑制剂Bcl-2的表达下调，但这种趋势通过单一和组合治疗得以逆转。此外，针刺组和康复组的TNF-α、IL-1β和ROS含量均显著低于模型组，但高于实验组。而SOD活性则相反。此外，与模型组相比，针刺、康复和实验组中BDNF、S100β和GFAP阳性细胞的比率显著增加，实验组中的比率最高。结论：本研究表明，头针结合运动疗法可通过降低促炎介质和活性氧、增加抗氧化酶SOD、神经营养因子BDNF和星形胶质细胞活性，有效对抗缺血性脑损伤。

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