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首页> 外文期刊>Journal of the mechanical behavior of biomedical materials >Micro-mechanical damage of needle puncture on bovine annulus fibrosus fibrils studied using polarization-resolved Second Harmonic Generation (P-SHG) microscopy
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Micro-mechanical damage of needle puncture on bovine annulus fibrosus fibrils studied using polarization-resolved Second Harmonic Generation (P-SHG) microscopy

机译：用偏振分辨的二次谐波产生（P-SHG）显微镜研究牛刺穿的针刺纤维纤维的微机械损伤

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摘要

Needle injection has been widely used in spinal therapeutic or diagnostic processes, such as discography. The use of needles has been suspected in causing mild disc degeneration which can lead to long-term back pain. However, the localised microscopic damage caused by needles has not been well studied. The local progressive damage on a microscopic level caused by needle punctures on the surface of bovine annulus fibrosus was investigated. Four different sizes of needle were used for the puncture and twenty-nine bovine intervertebral discs were studied. Polarization-resolved second harmonic generation and fluorescent microscopy were used to study the local microscopic structural changes in collagen and cell nuclei due to needle damage. Repeated 70 cyclic loadings at ?5% of axial strain were applied after the needle puncture in order to assess progressive damage caused by the needle. Puncture damage on annulus fibrosus were observed either collagen fibre bundles being pushed aside, being cut through or combination of both with part being lift or pushed in. The progressive damage was found less relevant to the needle size and more progressive damage was only observed using the larger needle. Two distinct populations of collagen, in which one was relatively more organised than the other population, were observed especially after the puncture from skewed distribution of polarization-SHG analysis. Cell shape was found rounder near the puncture site where collagen fibres were damaged.
机译：针头注射已广泛应用于脊柱治疗或诊断过程，如椎间盘造影术。使用针头被怀疑会导致轻度椎间盘退变，从而导致长期背痛。然而，针头造成的局部微观损伤尚未得到很好的研究。研究了针刺穿牛纤维环表面引起的局部进行性损伤。使用四种不同尺寸的针进行穿刺，并对29个牛椎间盘进行了研究。利用偏振分辨二次谐波和荧光显微镜研究了针头损伤引起的胶原和细胞核的局部微观结构变化。重复70次循环荷载？针穿刺后施加5%的轴向应变，以评估针造成的渐进性损伤。纤维环的穿刺损伤可观察到胶原纤维束被推到一边、被切断或两者结合，部分被提起或推入。发现渐进性损伤与针头大小的相关性较小，仅使用较大的针头观察到更多渐进性损伤。通过偏振光倍频分析的偏态分布，观察到两个不同的胶原蛋白群体，其中一个群体比另一个群体相对更有组织性，尤其是在穿刺后。在胶原纤维受损的穿刺部位附近发现细胞形状更圆。

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