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首页> 外文期刊>Journal of the Science of Food and Agriculture >Structural characterization of calcium-binding sunflower seed and peanut peptides and enhanced calcium transport by calcium complexes in Caco-2 cells
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Structural characterization of calcium-binding sunflower seed and peanut peptides and enhanced calcium transport by calcium complexes in Caco-2 cells

机译：CaCO-2细胞钙络合物钙结合葵花籽和花生肽的结构表征及增强钙转运

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BACKGROUND Peptide-Ca complexes can promote Ca absorption. The present study aimed to determine the transport mechanism and structural characteristics of sunflower seed and peanut peptides with high Ca binding capacity with respect to developing third-generation Ca supplements and functional food ingredients. RESULTS High Ca-binding fractions of 1-3 kDa sunflower seed peptide (SSP4) and >= 10 kDa peanut peptide (PP1) had higher amount of Ca transported than CaCl(2)and two hydrolyzed proteins in Caco-2 cells. SSP(4)and PP(1)were separated by Ca ion metal chelate affinity chromatography, and high Ca-binding fractions were observed for SSP4-P(2)and PP1-P-2. The amino acid sequences of SSP4-P(2)and PP1-P(2)were characterized by high-performance liquid chromatography-electrospray ionization-time of flight mass spectrometry. Seven and eight peptides were identified from SSP4-P(2)and PP1-P-2, respectively. These peptides had molecular weights ranging from 1500 Da to 2500 Da and a large number of characteristic amino acid sequences, such as EEEQQQ, EQ-QQQ-QQ, QQ-QQQQQ, E-EEE, EE-EEQ, RR, Q-QQ-QQQ, EE-EQ-EE-Q, QQ-QQQQ, and Q-QQQQ, where 'E' is glutamic acid and 'Q' is glutamine. CONCLUSION SSP(4)and PP(1)can promote Ca transport in Caco-2 cells without affecting cell permeability. The amino acid sequences of SSP4-P(2)and PP1-P(2)with high Ca-binding abilities contain characteristic sequences, such as continuous glutamic acid and glutamine, and have low molecular weights. (c) 2020 Society of Chemical Industry
机译：背景肽钙复合物能促进钙的吸收。本研究旨在确定向日葵种子和花生肽的转运机制和结构特征，以开发第三代钙补充剂和功能性食品成分。结果1-3 kDa葵花籽肽（SSP4）和>=10 kDa花生肽（PP1）的高钙结合组分在Caco-2细胞中的钙转运量高于CaCl（2）和两种水解蛋白。用钙离子金属螯合亲和层析分离SSP（4）和PP（1），观察到SSP4-P（2）和PP1-P-2的高钙结合组分。采用高效液相色谱-电喷雾电离飞行时间质谱法对SSP4-P（2）和PP1-P（2）的氨基酸序列进行了表征。分别从SSP4-P（2）和PP1-P-2中鉴定出七个和八个肽。这些肽的分子量从1500 Da到2500 Da不等，并具有大量特征性氨基酸序列，如Eeqqq、EQ-QQQ-QQ、QQ-QQQQ、E-EEE、EE-EEQ、RR、Q-QQ-QQ、EE-EQ-EE-Q、QQ-QQQ和Q-QQQ，其中“E”是谷氨酸，“Q”是谷氨酰胺。结论SSP（4）和PP（1）能促进Caco-2细胞钙转运，但不影响细胞通透性。具有高钙结合能力的SSP4-P（2）和PP1-P（2）的氨基酸序列包含特征序列，例如连续的谷氨酸和谷氨酰胺，并且具有低分子量。（c） 2020年化学工业学会

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