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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >GDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in rats
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GDNF Schwann cells in hydrogel scaffolds promote regional axon regeneration, remyelination and functional improvement after spinal cord transection in rats

机译：水凝胶支架中的GDNF Schwann细胞促进大鼠脊髓横划伤后的区域轴突再生，重新髓鞘，官能改善

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摘要

Positively-charged oligo[poly(ethylene glycol)fumarate] (OPF+) is a biodegradable hydrogel used for spinal cord injury repair. We compared scaffolds containing primary Schwann cells (SCs) to scaffolds delivering SCs genetically modified to secrete high concentrations of glial cell-derived neurotrophic factor (GDNF). Multichannel OPF+ scaffolds loaded with SCs or GDNF-SCs were implanted into transected rat spinal cords for 4 weeks. GDNF-SCs promoted regeneration of more axons into OPF+ scaffolds (2773.0 +/- 396.0) than primary SC OPF+ scaffolds (1666.0 +/- 352.2) (p = 0.0491). This increase was most significant in central and ventral-midline channels of the scaffold. Axonal remyelination was quantitated by stereologic analysis. Increased myelination of regenerating axons was observed in the GDNF-SC group. Myelinating cell and axon complexes were formed by host SCs and not by implanted cells or host oligodendrocytes. Fast Blue retrograde tracing studies determined the rostral-caudal directionality of axonal growth. The number of neurons that projected axons rostrally through the GDNF-SC scaffolds was higher (7929 +/- 1670) than in animals with SC OPF+ scaffolds (1069 +/- 241.5) (p 0.0001). The majority of ascending axons were derived from neurons located more than 15 mm from the scaffold-cord interface, and were identified to be lumbosacral intraspinal motor neurons. Transected animals with GDNF-SC OPF+ scaffolds partially recovered locomotor function at weeks 3 and 4 following surgery. Copyright (C) 2017 John Wiley & Sons, Ltd.
机译：带正电的低聚富马酸聚乙二醇酯（OPF+）是一种用于脊髓损伤修复的可生物降解水凝胶。我们比较了含有原代雪旺细胞（SCs）的支架和含有转基因SCs以分泌高浓度胶质细胞源性神经营养因子（GDNF）的支架。将含有SCs或GDNF-SCs的多通道OPF+支架植入横断的大鼠脊髓内4周。GDNF-SCs促进更多轴突再生为OPF+支架（2773.0+/-396.0），而不是初级SC-OPF+支架（1666.0+/-352.2）（p=0.0491）。这种增加在支架的中央和腹侧中线通道中最为显著。轴突再髓鞘化通过体视学分析进行定量。GDNF-SC组再生轴突髓鞘形成增加。髓鞘细胞和轴突复合体由宿主SCs形成，而不是由植入细胞或宿主少突胶质细胞形成。快速蓝色逆行追踪研究确定了轴突生长的嘴尾方向性。通过GDNF-SC支架投射轴突的神经元数量（7929+/-1670）高于使用SC-OPF+支架的动物（1069+/-241.5）（p；0.0001）。大多数上行轴突来自距离支架-脊髓界面超过15mm的神经元，经鉴定为腰骶椎管内运动神经元。在术后3周和4周，用GDNF-SC OPF+支架横断的动物部分恢复了运动功能。版权所有（C）2017约翰威利父子有限公司。

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