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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Generation and characterization of highly purified canine Schwann cells from spinal nerve dorsal roots as potential new candidates for transplantation strategies
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Generation and characterization of highly purified canine Schwann cells from spinal nerve dorsal roots as potential new candidates for transplantation strategies

机译：从脊神经背根的高度纯化的犬氏氏细胞的产生与表征作为移植策略的潜在新候选者

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Schwann cells are promising candidates for transplantation strategies in the central nervous system by promoting axonal regeneration. The dog represents a translational model for human spinal cord injury (SCI) for studies with new repair strategies after intervertebral disk herniation (IVDH). To overcome the necessity for an additional surgical procedure, for the first time a protocol for the isolation and purification of canine Schwann cells from spinal nerve biopsies during standard hemilaminectomy in IVDH-affected paraplegic dogs for potential transplantation has been developed. Purity was assessed by flow cytometry. The results were compared with biopsies from dogs without SCI. Within 26 +/- 4days, 90.2 +/- 8.8% p75 neurotrophin receptor (p75(NTR))-positive cells were achieved in IVDH dogs. The total cell count in acute/subacute and chronic IVDH (acute/subacute: 6.82 +/- 6.36 x 10(6); chronic: 2.29 +/- 2.00x10(6)) differed significantly (p = 0.0120) at the potential time point of transplantation. No differences in culture period and purity were detected between dogs with and without IVDH. Despite the small sample size and the altered environment, the isolation of Schwann cells was successful. Negative influences on isolation and purification due to potential pathological changes at the biopsy site of IVDH-diseased dogs were ruled out by comparison of Schwann cell pellets from diseased and control dogs. Finally, the functionality of Schwann cells from dogs with IVDH was outlined in co-culture experiments with canine dorsal root ganglion neurons. In conclusion, nerve root biopsies provide a sufficient number of highly purified and functional Schwann cells within a useful time period for novel therapeutic strategies in dogs with SCI.
机译：雪旺细胞通过促进轴突再生，有望成为中枢神经系统移植策略的候选细胞。这只狗代表了人类脊髓损伤（SCI）的平移模型，用于研究椎间盘突出症（IVDH）后的新修复策略。为了克服额外外科手术的必要性，首次在IVDH受影响的截瘫犬进行标准半椎板切除术期间，制定了从脊髓神经活检中分离和纯化犬雪旺细胞的方案，用于潜在的移植。通过流式细胞术评估纯度。研究结果与未患脊髓损伤的狗的活检结果进行了比较。在26+/-4天内，IVDH犬的p75神经营养素受体（p75（NTR））阳性细胞达到90.2+/-8.8%。急性/亚急性和慢性IVDH中的细胞总数（急性/亚急性：6.82+/-6.36 x 10（6）；慢性：2.29+/-2.00x10（6））在移植的潜在时间点存在显著差异（p=0.0120）。在有和没有IVDH的狗之间，未检测到培养周期和纯度的差异。尽管样本量很小，环境也发生了改变，但施万细胞的分离还是成功的。通过比较病犬和对照犬的雪旺细胞颗粒，排除了IVDH病犬活检部位潜在病理变化对分离和纯化的负面影响。最后，在与狗背根神经节神经元的共培养实验中概述了患有IVDH的狗的雪旺细胞的功能。总之，神经根活检可在一段有用的时间内提供足够数量的高纯度和功能性雪旺细胞，用于SCI犬的新治疗策略。

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