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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >3D composite engineered using supercritical CO2 decellularized porcine cartilage scaffold, chondrocytes, and PRP: Role in articular cartilage regeneration
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3D composite engineered using supercritical CO2 decellularized porcine cartilage scaffold, chondrocytes, and PRP: Role in articular cartilage regeneration

机译：3D复合材料设计使用超临界CO2脱细胞软骨软骨脚手架，软骨细胞和PRP：在关节软骨再生中的作用

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At present, no definitive treatment for articular cartilage defects has been perfected. Most of the previous treatments involved multiple drilling and microfracture over defect sites with repair-related substances, which poses a limited therapeutic effect. End-stage therapy includes artificial knee joint replacement. In this study, we prepared a novel decellularized natural cartilage scaffold from porcine articular cartilage by supercritical CO2 extraction technology and three-dimensional (3D) composites made using decellularized porcine cartilage graft (dPCG) as scaffolds, platelet-rich plasma (PRP), thrombin as signals and chondrocytes as cells for the treatment of articular cartilage defects. In this study, in vitro and in vivo cartilage regeneration and the expression of chondrogenic markers were examined. Decellularized cartilage graft (dPCG) was evaluated for the extent of cell and DNA removal. Residual cartilage ECM structure was confirmed to be type II collagen by SDS PAGE and immunostaining. The new 3D composite with dPCG (100 mg and 2 x 10(6) chondrocytes) scaffold promotes chondrogenic marker expression in vitro. We found that the in vivo 3D composite implanted cartilage defect showed significant regeneration relative to the blank and control implant. Immunohistochemical staining showed increase of expression including Collagen type II and aggrecan in 3D composite both in vitro and in vivo studies. In this study, the bioengineered 3D composite by combining dPCG scaffold, chondrocytes, and PRP facilitated the chondrogenic marker expression in both in vitro and in vivo models with accelerated cartilage regeneration. This might serve the purpose of clinical treatment of large focal articular cartilage defects in humans in the near future.
机译：目前，关节软骨缺损尚无明确的治疗方法。以前的大多数治疗方法都涉及在缺损部位进行多次钻孔和微骨折，并含有修复相关物质，因此治疗效果有限。终末期治疗包括人工膝关节置换术。在本研究中，我们采用超临界CO2萃取技术从猪关节软骨中制备了新型脱细胞天然软骨支架，并以脱细胞猪软骨移植物（dPCG）为支架，富含血小板血浆（PRP）、凝血酶为信号，软骨细胞为细胞制备了三维复合材料，用于治疗关节软骨缺损。在这项研究中，我们检测了体外和体内软骨再生以及软骨生成标记物的表达。评估脱细胞软骨移植（dPCG）的细胞和DNA去除程度。SDS-PAGE和免疫组化证实残留的软骨ECM结构为II型胶原。含有dPCG（100 mg和2 x 10（6）软骨细胞）支架的新型3D复合材料在体外促进软骨生成标记物的表达。我们发现，在体内3D复合植入软骨缺损表现出明显的再生相对于空白和控制植入物。免疫组化染色显示3D复合物在体外和体内的表达增加，包括II型胶原和聚集蛋白聚糖。在这项研究中，结合dPCG支架、软骨细胞和PRP的生物工程3D复合材料促进了软骨生成标记物在体外和体内模型中的表达，加速了软骨再生。这可能有助于在不久的将来临床治疗人类的大型局灶性关节软骨缺损。

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