A uniform expression library for the exploration of FOX transcription factor biology

Moparthi Lavanya; Koch Stefan

首页> 外文期刊>Differentiation: The Journal of the International Society of Differentiation >A uniform expression library for the exploration of FOX transcription factor biology

【24h】

A uniform expression library for the exploration of FOX transcription factor biology

机译：氟于狐狸转录因子生物学探索的统一表达库

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Forkhead box (FOX) family transcription factors play essential roles in development, tissue homeostasis, and disease. Although the biology of several FOX proteins has been studied in depth, it is unclear to what extent these findings apply to even closely related family members, which frequently exert overlapping but non-redundant functions. To help address this question, we have generated a uniform, ready-to-use expression library of all 44 human FOX transcription factors with a convenient peptide tag for parallel screening assays. In addition, we have generated multiple universal forkhead box reporter plasmids, which can be used to monitor the transcriptional activity of most FOX proteins with high fidelity. As a proof-of-principle, we use our plasmid library to identify the DNA repair protein XRCC6/Ku70 as a selective FOX interaction partner and regulator of FOX transcriptional activity. We believe that these tools, which we make available via the Addgene plasmid repository, will considerably expedite the investigation of FOX protein biology.

机译：FOX家族转录因子在发育、组织内稳态和疾病中起着重要作用。虽然已经对几种狐狸蛋白的生物学进行了深入的研究，但尚不清楚这些发现在多大程度上适用于即使是密切相关的家族成员，这些家族成员经常发挥重叠但非冗余的功能。为了帮助解决这个问题，我们制作了一个统一的、现成的表达文库，包含所有44种人类FOX转录因子，带有一个方便的肽标签，用于平行筛选分析。此外，我们还制备了多个通用叉头盒报告质粒，可用于高保真地监测大多数FOX蛋白的转录活性。作为原理证明，我们使用我们的质粒文库鉴定DNA修复蛋白XRCC6/Ku70作为选择性FOX相互作用伙伴和FOX转录活性调节器。我们相信，我们通过Addgene质粒库提供的这些工具将大大加快FOX蛋白质生物学的研究。

著录项

来源
《Differentiation: The Journal of the International Society of Differentiation》 |2020年第1期|共7页
作者
Moparthi Lavanya; Koch Stefan;
展开▼
作者单位

Linkoping Univ Dept Biomed &

Clin Sci BKV Linkoping Sweden;

Linkoping Univ Dept Biomed &

Clin Sci BKV Linkoping Sweden;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类细胞生物学;
关键词
Forkhead box; Luciferase reporter; Transcription; DNA repair; Ku complex;

机译：叉头箱;荧光素酶报告;转录;DNA修复;古情结;

相似文献

外文文献
中文文献
专利

1. Expressions of transcription factors Smad4 and NF-κB in preeclampsia placenta tissue and exploration of its relationship with expressions of apoptotic and invasive genes [J] . Li Xia 海南医科大学学报（英文版） . 2015,第010期
2. Oligodendrocyte transcription factor 1 overexpression promotes oligodendrocyte transcription factor 2 expression in the brains of neonatal rats exposed to hypoxia****☆ [J] . Lijun Yang, Hong Cui, Aijun Yang, 中国神经再生研究（英文版） . 2011,第034期
3. Growth-Regulating Factors (GRFs): A Small Transcription Factor Family with Important Functions in Plant Biology [J] . Mohammad Amin Omidbakhshfard, Sebastian Proost, Ushio Fujikura, 分子植物（英文版） . 2015,第007期
4. Expression of transcription factor Klf8 in lung cancer tissue and the biological effect of downregulation of Klf8 expression in lung cancer cell lines [J] . Xuan-Hong Yi, Jing Wang 海南医科大学学报（英文版） . 2016,第002期
5. Identification and expression profiles of Fox transcription factors in the Yesso scallop (Patinopecten yessoensis) [J] . Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function . 2020,第期

机译：Yesso扇贝（Patinopecten Yessoensis）的狐狸转录因子识别和表达谱
6. Comprehensive Expression Analysis Suggests Functional Overlapping of Human FOX Transcription Factors in Cancer [J] . Ya-Li Zhang, Feng-Ting Sun, Ze Zhang, Asian Pacific Journal of Cancer Prevention . 2015,第23期

机译：综合表达分析表明人类狐狸转录因子在癌症中的功能重叠
7. Fox tales: Regulation of gonadotropin gene expression by forkhead transcription factors [J] . ThackrayV.G. Molecular and Cellular Endocrinology . 2014,第1a2期

机译：狐狸的故事：叉头转录因子对促性腺激素基因表达的调节
8. Transcriptome (RNA-seq) analysis of human salivary gland cells with exogenous expression of human pancreas beta cells transcription factors PDX1, MAFA, NGN3 [C] . Olga Brovkina, Yulia Kolesova, Mikhail Borisov, Conference on Cognitive Sciences, Genomics and Bioinformatics . 2020

机译：外源表达人胰腺β细胞转录因子PDX1，MAFA，NGN3的人唾液腺细胞的转录组（RNA-seq）分析
9. Exploration of the cis-elements and trans-factors involved in post-transcriptional mechanisms of the gene atpI in chloroplast gene expression [D] . Smithers, Emily Rose 2011

机译：探索叶绿体基因表达中atpI基因转录后机制的顺式和反式因子
10. Fox Tales: Regulation of Gonadotropin Gene Expression by Forkhead Transcription Factors [O] . Varykina G. Thackray -1

机译：狐狸的故事：叉头转录因子对促性腺激素基因表达的调节。
11. Comprehensive Expression Analysis Suggests Functional Overlapping of Human FOX Transcription Factors in Cancer [O] . Ya-Li Zhang, Feng-Ting Sun, Ze Zhang, 2015

机译：综合表达分析表明人类狐狸转录因子在癌症中的功能重叠
12. Promoter Switching and Transcription Factor Usage During Breast Adipocyte Differentiation: Role in Aromatase Expression and Activity [R] . Mizinga, K. M. 2005

机译：乳腺脂肪细胞分化过程中启动子转换和转录因子的使用：在芳香化酶表达和活性中的作用

A uniform expression library for the exploration of FOX transcription factor biology

摘要

著录项

相似文献

相关主题

期刊订阅