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首页> 外文期刊>Nucleic Acid Therapeutics >Comparison of Peptide- and Lipid-Based Delivery of miR-34a-5p Mimic into PPC-1 Cells
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Comparison of Peptide- and Lipid-Based Delivery of miR-34a-5p Mimic into PPC-1 Cells

机译：MiR-34a-5p基于肽和脂质的递送到PPC-1细胞中的肽和脂质递送的比较

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摘要

The microRNA (miRNA) microRNA-34a (miR-34a) regulates a number of genes involved in cell cycle control and is therefore considered to have a high therapeutic potential. MiR-34a expression is often down-regulated in cancer cells and its restoration has been shown to exert a tumor-suppressive effect. However, effective and safe delivery of synthetic miRNA analogs into cancer cells remains a challenge. The aim of this study was to evaluate cell-penetrating peptide PepFect (PF) 14 as a carrier for delivery of miR-34a-5p into human primary prostate carcinoma-1 (PPC-1) cells. Using microarray expression analysis, we identified a total of 3,283 (1,744 upregulated and 1,539 downregulated) differentially expressed genes in PF14: miR-34a-5p-transfected cells. In comparison, miR-34a-5p delivery with the commercially available lipid-based reagent siPORT-NeoFX (siPORT) had less robust effects on differential expression and affected fewer genes significantly (90 upregulated and 91 downregulated genes). Functional annotation revealed significant enrichment for downregulated genes in processes and pathways associated with the cell cycle and proliferation regulation in PF14: miR-34a-5p-transfected cells. Five genes (ARHGAP1, AXL, CDC25A, FOSL1, and PDGFRA) were identified and validated as relevant quantitative real-time polymerase chain reaction-based markers of miR-34a-5p transfection efficiency. Our experiments revealed novel potential miR-34a-5p targets and demonstrate that PF14 is a reliable transfection reagent for miRNA mimics characterized by high efficiency and low toxicity relative to lipid-based reagents.
机译：MicroRNA（MIRNA）MicroRNA-34A（MIR-34A）规范细胞周期控制中基因的数量，并被认为具有较高的治疗潜力。MIR-34A Expression是一种在癌症细胞中被调控的表达方式，它的恢复已经被展示出一种肿瘤超压效应。但是，将合成mirna分析引入癌症细胞仍然是一个挑战。The AIM of this study was to evaluate cell-penetrating peptide pepfect（PF）14 as a carrier for delivery of mir-34a-5p into human primary prostate carcinoma-1（PPC-1）cells.使用Microarray Expression Analysis，我们确定了PF14:MIR-34A-5P-Transfected cells中3283（1744次更新和1539次下载）不同表达基因的总数。在比较中，MIR-34A-5P与商业上可用的LIPID-based Reagent Siport-NEOFX（Siport）在不同的表达和影响的能量生成信号（90个upregulated和91个downregulated genes）上具有最小的robust效应。PF14:MIR-34A-5P-transfected cells.五个基因（Arhgap1、AXL、CDC25A、FOSL1和PDGFRA）是MIR-34A-5P转换效率的相关量化实时多指标链路响应基准市场。我们的经验揭示了MIR-34A-5P潜在目标和演示，PF14是一个可靠的传输响应MIRNA MIMICS的特点，具有高效率和低毒性相关的lipid-based响应。

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