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首页> 外文期刊>Biochemistry >Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents
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Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents

机译：来自Saposin蛋白质和支链洗涤剂的稳定Picodisc组件

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摘要

Methods for maintaining membrane proteins in their native state after removal from the lipid bilayer are essential for the study of this important class of biomacromolecules. Common solubilization strategies range from the use of detergents to more complex systems that involve a polypeptide working in concert with lipids or detergents, such as nanodiscs, picodiscs, and peptidiscs, in which an engineered protein or synthetic peptide surrounds the membrane protein along with a lipid sheath. Picodiscs employ the protein saposin A, which naturally functions to facilitate lipid degradation in the lysozome. Saposin A–amphiphile complexes therefore tend to be most stable at acidic pH, which is not optimal for most membrane protein applications. In search of new picodisc assemblies, we have explored pairings of saposin A or other saposin proteins with a range of detergents, and we have identified a number of combinations that spontaneously co-assemble at neutral pH. The resulting picodiscs are stable for weeks and have been characterized by size-exclusion chromatography, native mass spectrometry, and small angle X-ray scattering. The new assemblies are formed by double-tail detergents rather than more traditional single-tail detergents; the double-tail detergents can be seen as structurally intermediate between single-tail detergents and common lipids. In addition to saposin A, an engineered variant of saposin B (designated saposin B~(W)) forms picodisc assemblies. These findings provide a framework for future efforts to solubilize membrane proteins with multiple picodisc systems that were previously unknown.
机译：从脂质双层中去除膜蛋白后，保持膜蛋白处于其天然状态的方法对于研究这类重要的生物大分子至关重要。常见的增溶策略包括从使用洗涤剂到更复杂的系统，其中涉及与脂质或洗涤剂协同工作的多肽，例如纳米盘、picodiscs和肽iscs，其中工程蛋白或合成肽与脂质鞘一起包围膜蛋白。Picodiscs利用蛋白saposin A，其天然功能是促进溶菌酶中的脂质降解。因此，Saposin A–两亲性复合物在酸性pH下往往最稳定，这对于大多数膜蛋白应用来说并不理想。为了寻找新的picodisc组装体，我们探索了saposin A或其他saposin蛋白质与一系列洗涤剂的配对，并确定了一些在中性pH下自发共组装的组合。产生的picodisc稳定数周，并通过尺寸排除色谱、自然质谱和小角X射线散射进行了表征。新组件由双尾洗涤剂组成，而不是更传统的单尾洗涤剂；双尾洗涤剂在结构上介于单尾洗涤剂和普通油脂之间。除了saposin A之外，saposin B的一种工程变体（命名为saposin B~（W））形成picodisc组装体。这些发现为将来利用多个以前未知的picodisc系统溶解膜蛋白提供了一个框架。

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  • 来源
    《Biochemistry》 |2021年第14期|共12页
  • 作者

    Kathleen W. Kurgan; Bifan Chen; Kyle A. Brown; Paulo Falco Cobra; Xinyu Ye; Ying Ge; Samuel H. Gellman;

  • 作者单位

    Department of Chemistry University of Wisconsin?Madison;

    Department of Chemistry ? Department of Cell and Regenerative Biology ? Human Proteomics Program ? University of Wisconsin?Madison;

    Department of Chemistry ? Department of Cell and Regenerative Biology ? Human Proteomics Program ? University of Wisconsin?Madison;

    National Magnetic Resonance Facility at Madison University of Wisconsin?Madison;

    Department of Chemistry University of Wisconsin?Madison;

    Department of Chemistry ? Department of Cell and Regenerative Biology ? Human Proteomics Program ? University of Wisconsin?Madison;

    Department of Chemistry University of Wisconsin?Madison;

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  • 正文语种 eng
  • 中图分类 生物化学;
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