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首页> 外文期刊>Brain research >Ultrastructural evidence for mu and delta opioid receptors at noradrenergic dendrites and glial profiles in the cat locus coeruleus
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Ultrastructural evidence for mu and delta opioid receptors at noradrenergic dendrites and glial profiles in the cat locus coeruleus

机译：Mu和Delta阿片受体在猫基因士群岛的麦芽糖蛋白质受体的超微结构证据

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The Locus Coeruleus (LC) is a pontine nucleus involved in many physiological processes, including the control of the sleep/wake cycle (SWC). At cellular level, the LC displays a high density of opioid receptors whose activation decreases the activity of LC noradrenergic neurons. Also, microinjections of morphine administered locally in the LC of the cat produce sleep associated with synchronized brain activity in the electroencephalogram (EEG). Even though much of the research on sleep has been done in the cat, the subcellular location of opioid receptors in the LC and their relationship with LC noradrenergic neurons is not known yet in this species. Therefore, we conducted a study to describe the ultrastructural localization of mu-opioid receptors (MOR), delta-opioid receptors (DOR) and tyrosine hydroxylase (TH) in the cat LC using high resolution electron microscopy double-immunocytochemical detection. MOR and DOR were localized mainly in dendrites (45% and 46% of the total number of profiles respectively), many of which were noradrenergic (35% and 53% for MOR and DOR, respectively). TH immunoreactivity was more frequent in dendrites (65% of the total number of profiles), which mostly also expressed opioid receptors (58% and 73% for MOR and DOR, respectively). Because the distribution of MORs and DORs are similar, it is possible that a substantial sub-population of neurons co-express both receptors, which may facilitate the formation of MOR-DOR heterodimers. Moreover, we found differences in the cat subcellular DOR distribution compared with the rat. This opens the possibility to the existence of diverse mechanisms for opioid modulation of LC activity.
机译：蓝斑（LC）是脑桥核，参与许多生理过程，包括睡眠/觉醒周期（SWC）的控制。在细胞水平上，LC显示出高密度的阿片受体，其激活降低了LC去甲肾上腺素能神经元的活性。此外，在猫的LC局部注射吗啡会产生与脑电图（EEG）同步脑活动相关的睡眠。尽管大多数关于睡眠的研究都是在猫身上进行的，但在这个物种中，阿片受体在LC中的亚细胞位置及其与LC去甲肾上腺素能神经元的关系尚不清楚。因此，我们进行了一项研究，用高分辨率电子显微镜双免疫细胞化学检测描述猫LC中μ阿片受体（MOR）、δ阿片受体（DOR）和酪氨酸羟化酶（TH）的超微结构定位。MOR和DOR主要定位于树突中（分别占图谱总数的45%和46%），其中许多是去甲肾上腺素能的（MOR和DOR分别占35%和53%）。TH免疫反应在树突中更为常见（占图谱总数的65%），树突主要也表达阿片受体（MOR和DOR分别为58%和73%）。由于MOR和DOR的分布相似，可能有大量神经元亚群共同表达这两种受体，这可能促进MOR-DOR异二聚体的形成。此外，我们发现猫的亚细胞DOR分布与大鼠相比存在差异。这为阿片类药物调节LC活性的多种机制的存在提供了可能性。

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