Multi-plane confocal microscopy with multiplexed volume holographic gratings [Invited]

Chia Chou-Min; Vyas Sunil; Wu Tso-Hua; Yeh J. Andrew; Luo Yuan

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Multi-plane confocal microscopy with multiplexed volume holographic gratings [Invited]

机译：多平面共聚焦显微镜与多路复用卷全息放射[邀请]

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A volume holographic (VHG) grating-based multi-plane differential confocal microscopy (DCM) is proposed for axial scan-free imaging. Also, we briefly reviewed our previous works on volume holographic-based confocal imaging. We show that without degrading imaging performance, it is possible to simultaneously obtain two depth-resolved optically sectioned images with improved axial resolution using multi-plane DCM. The performance of our multi-plane DCM was evaluated by measuring the surface profile of a silicon micro-hole array with depths separation around 10 pin. The axial sensitivity of the system is around 25 nm. Our system has the advantages of multi-plane imaging with high axial sensitivity and high optical sectioning ability. Our method can be used for reflective surface profiling and multi-plane fluorescence imaging. The present methods may find important applications in surface metrology for label-free biological samples, as well as industrial applications. (C) 2021 Optical Society of America

机译：提出了一种基于体全息（VHG）光栅的多平面微分共焦显微镜（DCM），用于轴向无扫描成像。此外，我们还简要回顾了我们以前在体全息共焦成像方面的工作。我们表明，在不降低成像性能的情况下，使用多平面DCM可以同时获得两个深度分辨率的光学切片图像，并提高轴向分辨率。通过测量深度间隔约为10针的硅微孔阵列的表面轮廓，评估了我们的多平面DCM的性能。该系统的轴向灵敏度约为25 nm。我们的系统具有多平面成像的优点，轴向灵敏度高，光学切片能力强。我们的方法可用于反射表面轮廓和多平面荧光成像。目前的方法可能在无标签生物样品的表面计量以及工业应用中找到重要应用。（2021）美国光学学会

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《Applied optics》 |2021年第10期|共10页
作者
Chia Chou-Min; Vyas Sunil; Wu Tso-Hua; Yeh J. Andrew; Luo Yuan;
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作者单位

Natl Taiwan Univ Inst Med Device &

Imaging Taipei 10051 Taiwan;

Natl Taiwan Univ Inst Med Device &

Imaging Taipei 10051 Taiwan;

Natl Taiwan Univ Inst Med Device &

Imaging Taipei 10051 Taiwan;

Natl Tsing Hua Univ Dept Power Mech Engn Hsinchu 30013 Taiwan;

Natl Taiwan Univ Inst Med Device &

Imaging Taipei 10051 Taiwan;

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正文语种 eng
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机译：光热折射玻璃中多重体布拉格光栅的全息记录及应用。

Multi-plane confocal microscopy with multiplexed volume holographic gratings [Invited]

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