• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied optics >Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy
【24h】

Real-time quantitative phase imaging by single-shot dual-wavelength off-axis digital holographic microscopy

机译：单次双波长脱轴数字全息显微镜实时定量相位成像

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A single-shot dual-wavelength digital holographic microscopy with an adjustable off-axis configuration is presented, which helps realize real-time quantitative phase imaging for living cells. With this configuration, two sets of interference fringes corresponding to their wavelengths can be flexibly recorded onto one hologram in one shot. The universal expression on the dual-wavelength hologram recorded under any wave vector orientation angles of reference beams is given. To avoid as much as possible the effect of zero-order spectrum, we can flexibly select their carry frequencies for the two wavelengths using this adjustable off-axis configuration, according to the distribution feature of object's spatial-frequency spectrum. This merit is verified by a quantitative phase imaging experiment for the microchannel of a microfluidic chip. The reconstructed phase maps of living onion epidermal cells exhibit cellular internal life activities, for the first time to the best of our knowledge, vividly displaying the progress of the nucleus, cell wall, cytoskeleton, and the substance transport in microtubules inside living cells. These imaging results demonstrate the availability and reliability of the presented method for real-time quantitative phase imaging. (C) 2021 Optical Society of America
机译：本文介绍了一种离轴可调的单次双波长数字全息显微镜，它有助于实现活细胞的实时定量相位成像。通过这种配置，两组与它们的波长对应的干涉条纹可以在一次拍摄中灵活地记录到一张全息图上。给出了双波长全息图在参考光任意波矢方位角下的通用表达式。为了尽可能避免零阶谱的影响，我们可以根据物体空间频谱的分布特征，使用这种可调的离轴配置，灵活地选择两个波长的载波频率。这一优点通过微流控芯片微通道的定量相位成像实验得到了验证。据我们所知，重建的洋葱表皮活细胞相位图首次展示了细胞内部的生命活动，生动地展示了细胞核、细胞壁、细胞骨架以及活细胞内微管中物质运输的进展。这些成像结果证明了所提出的实时定量相位成像方法的有效性和可靠性。（2021）美国光学学会

著录项

  • 来源
    《Applied optics》 |2021年第15期|共8页
  • 作者

    Huang Meng; Qin Hongpeng; Jiang Zhuqing;

  • 作者单位

    Beijing Univ Technol Coll Phys &

    Optoelect Fac Sci Beijing 100124 Peoples R China;

    Beijing Univ Technol Coll Phys &

    Optoelect Fac Sci Beijing 100124 Peoples R China;

    Beijing Univ Technol Coll Phys &

    Optoelect Fac Sci Beijing 100124 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 应用;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号