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Open-source, cost-effective, portable, 3D-printed digital lensless holographic microscope

机译：开源，经济高效，便携式，3D印刷数字透镜全息显微镜

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摘要

In this work, the design, construction, and testing of the most cost-effective digital lensless holographic microscope to date are presented. The architecture of digital lensless holographic microscopy (DLHM) is built by means of a 3D-printed setup and utilizing off-the-shelf materials to produce a DLHM microscope costing US$52.82. For the processing of the recorded in-line holograms, an open-source software specifically developed to process this type of recordings is utilized. The presented DLHM setup has all the degrees of freedom needed to achieve different fields of view, levels of spatial resolution, and 2D scanning of the sample. The feasibility of the presented platform is tested by imaging non-bio and bio samples; the resolution test targets, a section of the head of a Drosophila melanogaster fly, red blood cells, and cheek cells are imaged on the built microscope. (C) 2020 Optical Society of America
机译：本文介绍了迄今为止最具成本效益的数字无透镜全息显微镜的设计、制造和测试。数字无透镜全息显微镜（DLHM）的体系结构是通过3D打印设置和使用现成材料制作的，成本为52.82美元。为了处理记录的在线全息图，使用了专门为处理此类记录而开发的开源软件。所提出的DLHM装置具有实现不同视场、空间分辨率和样本二维扫描所需的所有自由度。通过对非生物和生物样品的成像，验证了该平台的可行性；分辨率测试的目标是黑腹果蝇头部的一部分、红细胞和脸颊细胞，这些都在内置的显微镜上成像。（C） 2020美国光学学会

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