Influence of noise-reduction techniques in sparse-data sample rotation tomographic imaging

Balasubramani Vinoth; Montresor Silvio; Tu Han-Yen; Huang Chung-Hsuan; Picart Pascal; Cheng Chau-Jern

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Influence of noise-reduction techniques in sparse-data sample rotation tomographic imaging

机译：稀疏数据样本旋转断层切断成像中降噪技术的影响

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Data acquisition and processing is a critical issue for high-speed applications, especially in three-dimensional live cell imaging and analysis. This paper focuses on sparse-data sample rotation tomographic reconstruction and analysis with several noise-reduction techniques. For the sample rotation experiments, a live Candida rugosa sample is used and controlled by holographic optical tweezers, and the transmitted complex wavefronts of the sample are recorded with digital holographic microscopy. Three different cases of sample rotation tomography were reconstructed for dense angle with a step rotation at every 2 degrees, and for sparse angles with step rotation at every 5 degrees and 10 degrees. The three cases of tomographic reconstruction performance are analyzed with consideration for data processing using four noise-reduction techniques. The experimental results demonstrate potential capability in retaining the tomographic image quality, even at the sparse angle reconstructions, with the help of noise-reduction techniques. (C) 2021 Optical Society of America

机译：数据采集和处理是高速应用的关键问题，尤其是在三维活细胞成像和分析中。本文主要研究稀疏数据样本旋转层析重建和分析，以及几种降噪技术。在样品旋转实验中，使用活体念珠菌样品并用全息光镊控制，用数字全息显微镜记录样品的透射复波前。对三种不同情况的样本旋转断层扫描进行了重建，分别针对密集角度和稀疏角度，每2度进行一次阶梯旋转，每5度和10度进行一次阶梯旋转。考虑到使用四种降噪技术进行数据处理，分析了三种情况下的断层重建性能。实验结果表明，在降噪技术的帮助下，即使在稀疏角度重建中，仍有保持层析图像质量的潜在能力。（2021）美国光学学会

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《Applied optics》 |2021年第10期|共7页
作者
Balasubramani Vinoth; Montresor Silvio; Tu Han-Yen; Huang Chung-Hsuan; Picart Pascal; Cheng Chau-Jern;
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Natl Taiwan Normal Univ Inst Electroopt Engn Taipei 11677 Taiwan;

Le Mans Univ LAUM CNRS UMR 6613 Ave Olivier Messiaen F-72085 Le Mans 9 France;

Chinese Culture Univ Dept Elect Engn Taipei 11114 Taiwan;

Natl Taiwan Normal Univ Inst Electroopt Engn Taipei 11677 Taiwan;

Le Mans Univ LAUM CNRS UMR 6613 Ave Olivier Messiaen F-72085 Le Mans 9 France;

Natl Taiwan Normal Univ Inst Electroopt Engn Taipei 11677 Taiwan;

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Influence of noise-reduction techniques in sparse-data sample rotation tomographic imaging

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