Crocin inhibits urea-induced amyloid formation by bovine beta-lactoglobulin

Bodiga Vijaya Lakshmi; Kudle Madhukar Rao; Vemuri Praveen Kumar; Bodiga Sreedhar

首页> 外文期刊>New Journal of Chemistry >Crocin inhibits urea-induced amyloid formation by bovine beta-lactoglobulin

【24h】

Crocin inhibits urea-induced amyloid formation by bovine beta-lactoglobulin

机译：Crocin抑制尿布β-乳糖蛋白的尿素诱导的淀粉样蛋白形成

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

beta-Lactoglobulin (beta-LG), a major whey protein, is able to form amyloid fibrillar aggregates, when subjected to urea-induced denaturation at pH 7.0. Crocin, being a polar carotenoid, was used to investigate its influence on the urea-induced unfolding of beta-LG and formation of amyloid fibrils. Crocin was found to stabilize beta-LG structure against urea at pH 7.0 and subsequently inhibited the amyloid fibril formation when challenged with 5 M urea for 2-4 weeks at 37 degrees C, as observed by thioflavin T fluorescence, congo red binding and transmission electron microscopy. The inhibition by crocin on beta-LG amyloid formation in a concentration-dependent manner exhibited a clear correlation between the midpoint of urea denaturation and lag time. Crocin was found to form a complex with beta-LG with K-d of 4 x 10(-7) M and it could be considered a potential therapeutic agent in the treatment of protein aggregation phenomena.

机译：β-乳球蛋白（β-LG）是一种主要的乳清蛋白，在pH值为7.0的尿素诱导变性时，能够形成淀粉样纤维聚集体。藏红花素是一种极性类胡萝卜素，用于研究其对尿素诱导的β-LG去折叠和淀粉样纤维形成的影响。通过硫黄素T荧光、刚果红结合和透射电子显微镜观察，发现藏红花素在pH 7.0下对尿素稳定β-LG结构，随后在37℃下用5 M尿素激发2-4周时抑制淀粉样纤维的形成。藏红花苷对β-LG淀粉样蛋白形成的抑制呈浓度依赖性，表明尿素变性中点与滞后时间之间存在明显的相关性。发现藏红花苷与β-LG形成复合物，K-d为4 x 10（-7）M，可被认为是治疗蛋白质聚集现象的潜在治疗剂。

著录项

来源
《New Journal of Chemistry》 |2021年第5期|共8页
作者
Bodiga Vijaya Lakshmi; Kudle Madhukar Rao; Vemuri Praveen Kumar; Bodiga Sreedhar;
展开▼
作者单位

Osmania Univ Inst Genet Dept Biochem &

Mol Biol Hyderabad Telangana India;

Kakatiya Univ Dept Biochem Warangal Telangana India;

KL Univ Dept Biotechnol Vaddeswaram Andhra Pradesh India;

Forest Coll &

Res Inst Dept Basic &

Social Sci Siddipet Telangana India;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类化学;
关键词

相似文献

外文文献
中文文献
专利

1. The novel amyloid-beta peptide aptamer inhibits intracellular amyloid-beta peptide toxicity [J] . Xu Wang, Yi Yang, Mingyue Jia, 中国神经再生研究（英文版） . 2013,第001期
2. Targeting amyloid precursor protein shuttling and processing - long before amyloid beta formation [J] . Sage Arbor 中国神经再生研究（英文版） . 2017,第002期
3. Amyloid fibril formation by native and modified bovine beta-lactoglobulins proceeds through unfolded form of proteins: a comparative study. [J] . Ghadami SA, Khodarahmi R, Ghobadi S, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：天然和修饰的牛β-乳球蛋白形成淀粉样蛋白原纤维的过程是通过蛋白质的未折叠形式进行的：一项比较研究。
4. Amyloid fibril formation by native and modified bovine beta-lactoglobulins proceeds through unfolded form of proteins: a comparative study. [J] . Ghadami SA, Khodarahmi R, Ghobadi S, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：通过展开的蛋白质的蛋白质：对比例进行的淀粉样蛋白原纤维形成通过展开的蛋白质：比较研究。
5. Amyloid fibril formation by native and modified bovine beta-lactoglobulins proceeds through unfolded form of proteins: a comparative study. [J] . Ghadami SA, Khodarahmi R, Ghobadi S, Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena . 2011,第2a3期

机译：通过展开的蛋白质的蛋白质：对比例进行的淀粉样蛋白原纤维形成通过展开的蛋白质：比较研究。
6. KINETICS OF BOVINE AND HUMAN INSULIN AMYLOID FIBRIL FORMATION IN BULK AND IN THE PRESENCE OF SOLID/LIQUID INTERFACES [C] . Paulina Barranco Morales, Daniel Forciniti AIChE annual meeting . 2011

机译：体液中存在牛和人胰岛素淀粉淀粉原纤维形成的动力学
7. Inhibitors of amyloid formation by islet amyloid polypeptide. [D] . Meng, Fanling. 2010

机译：胰岛淀粉样蛋白多肽形成淀粉样蛋白的抑制剂。
8. The Ability of Rodent Islet Amyloid Polypeptide to Inhibit Amyloid Formation by Human Islet Amyloid Polypeptide Has Important Implications For the Mechanism of Amyloid Formation and the Design of Inhibitors. [O] . Ping Cao, Fanling Meng, Andisheh Abedini, -1

机译：啮齿动物胰岛淀粉样蛋白多肽以抑制人胰岛淀粉样蛋白多肽抑制淀粉样蛋白形成的能力对淀粉样蛋白形成和抑制剂设计的机理具有重要意义。
9. The protective effect of crocin on the amyloid fibril formation of aβ42 peptide in vitro [O] . Arezou Ghahghaei, S. Bathaie, Hoda Kheirkhah, 2013

机译：披肩对体外Aβ42肽淀粉样蛋白原纤维形成的保护作用
10. Amyloid Formation in Alzheimer's Disease: An Immunohistochemical Study [R] . Rozemuller-Kwakkel, J. M. 1990

机译：阿尔茨海默病中的淀粉样蛋白形成：免疫组化研究

Crocin inhibits urea-induced amyloid formation by bovine beta-lactoglobulin

摘要

著录项

相似文献

相关主题

期刊订阅