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Harnessing the nutritional potential of concentrated whey for enhanced galactose flux in fermentative yeast

机译：利用浓缩乳清的营养潜力在发酵酵母中增强半乳糖通量的浓缩乳清

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摘要

The primary effluent of Indian dairy industries, cheese whey, and its main component lactose is considered an environmental pollutant due to its high chemical and biological oxygen demand. The model fermentative yeast, Saccharomyces cerevisiae, does not possess the necessary enzyme to utilize lactose in whey. In the present study, metabolic engineering of wild type S. cerevisiae strain with CRISPR Cas9 system was used to construct a cell factory that can flux higher galactose from the concentrated whey medium. The improved utilization of galactose confers fermentative metabolism with improved ethanol production rate. The strain provided a high titer of the ethanol (0.32 +/- 0.007 g/g) with improved galactose flux from concentrated whey at the expense of the biomass, glycerol, and acetic acid. Our present study provides evidence that galactose to ethanol fermentation is a feasible alternative for industrial waste stream treatment in developing countries.
机译：印度乳品工业的主要废水奶酪乳清及其主要成分乳糖因其化学和生物需氧量高而被视为环境污染物。模型发酵酵母酿酒酵母不具备利用乳清中乳糖所需的酶。在本研究中，利用CRISPR Cas9系统对野生型酿酒酵母菌株进行代谢工程，以构建一个能够从浓缩乳清培养基中输送更高半乳糖的细胞工厂。半乳糖利用率的提高赋予了发酵代谢和乙醇产率的提高。该菌株提供了高滴度的乙醇（0.32+/-0.007 g/g），提高了浓缩乳清中的半乳糖通量，但以生物量、甘油和乙酸为代价。我们目前的研究表明，在发展中国家，半乳糖发酵制乙醇是一种可行的工业废水处理方法。

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