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首页> 外文期刊>LWT-Food Science & Technology >A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity
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A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity

机译：来自乳杆菌唾液金黄色葡萄球菌的新细菌霉素：分离，纯化，鉴定，抗菌和抗菌和抗菌活性

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摘要

Bacteriocins are known to inhibit various foodborne bacteria and their biofilms; however, few bacteriocins with activity against food-derived Staphylococcus aureus have been reported. Here, a novel bacteriocin (XJS01) from the Lactobacillus salivarius strain CGMCC2070 was obtained, purified, and extensively characterized. Molecular mass and amino acid composition of XJS01 were 666.31 Da and F-S-G-L-A-G-D, respectively. XJS01 inhibited the S. aureus strain 2612:1606BL1486 (S. aureus_26), which was originally isolated from chicken meat. Also, XJS01 showed good resistance to heat treatment, and was only susceptible to pepsin treatment. The minimum inhibitory concentration of XJS01 against S. aureus_26 was 9.85 mu g/mL, which is lower than previously reported levels for most described bacteriocins. Furthermore, exposure to XJS01 also decreased viability and significantly inhibited (p < 0.05) planktonic S. aureus_26 cells. Biofilm formation of S. aureus_26 was also significantly inhibited (p < 0.05). The results of electron microscopy showed that XJS01 impaired membrane permeability in S. aureus_26, caused leakage of cytoplasmic content, and led to cell deformation. In summary, this study identifies the bacteriocin XJS01 as a potentially candidate for the control of S. aureus in foods in either planktonic or biofilm states.
机译：细菌素已知能抑制各种食源性细菌及其生物膜；然而，很少有对食物源性金黄色葡萄球菌具有活性的细菌素被报道。在此，从唾液乳杆菌菌株CGMCC2070中获得了一种新的细菌素（XJS01），并对其进行了纯化和广泛表征。XJS01的分子量和氨基酸组成分别为666.31 Da和F-S-G-L-A-G-D。XJS01抑制最初从鸡肉中分离的金黄色葡萄球菌菌株2612:1606BL1486（S.aureus_26）。此外，XJS01对热处理表现出良好的耐受性，仅对胃蛋白酶处理敏感。XJS01对金黄色葡萄球菌26的最低抑制浓度为9.85μg/mL，低于之前报道的大多数细菌素的水平。此外，暴露于XJS01也会降低存活率，并显著抑制（p<0.05）浮游金黄色葡萄球菌26细胞。金黄色葡萄球菌（S.aureus26）的生物膜形成也受到显著抑制（p<0.05）。电镜结果显示，XJS01破坏了金黄色葡萄球菌26的膜通透性，导致细胞质内容物泄漏，并导致细胞变形。总之，本研究确定细菌素XJS01可能是控制浮游或生物膜状态下食品中金黄色葡萄球菌的候选菌株。

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