Wavelength-scanning lensfree on-chip microscopy for wide-field pixel-super-resolved quantitative phase imaging

Wu Xuejuan; Sun Jiasong; Zhang Jialin; Lu Linpeng; Chen Rong; Chen Qian; Zuo Chao

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Wavelength-scanning lensfree on-chip microscopy for wide-field pixel-super-resolved quantitative phase imaging

机译：波长扫描透过镜片上的片上显微镜显微镜，用于宽场像素超级定量相位成像

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摘要

We propose a lensfree on-chip microscopy approach for wide-field quantitative phase imaging (QPI) based on wave-length scanning. Unlike previous methods, we found that a relatively large-range wavelength diversity not only provides information to overcome spatial aliasing of the image sensor but also creates sufficient diffraction variations that can be used to achieve motion-free, pixel-super-resolved phase recovery. Based on an iterative phase retrieval and pixel-super-resolution technique, the proposed wavelength-scanning approach uses only eight undersampled holograms to achieve a half-pitch lateral resolution of 691 nm across a large field-of-view of 29.85 mm(2), surpassing 2.41 times the theoretical Nyquist-Shannon sampling resolution limit imposed by the pixel size of the sensor (1.67 mu m). We confirmed the effectiveness of this technique in QPI and resolution enhancement by measuring the benchmark quantitative phase microscopy target. We also showed that this method can trackHeLa cell growth within an incubator, revealing cellular morphologies and subcellular dynamics of a large cell population over an extended period of time. (C) 2021 Optical Society of America

机译：我们提出了一种基于波长扫描的宽场定量相位成像（QPI）无透镜片上显微镜方法。与以前的方法不同，我们发现，相对较大的波长范围多样性不仅提供了克服图像传感器空间混叠的信息，而且还产生了足够的衍射变化，可用于实现无运动、像素超分辨率的相位恢复。基于迭代相位恢复和像素超分辨率技术，提出的波长扫描方法仅使用八个欠采样全息图，在29.85 mm（2）的大视场上实现691 nm的半节距横向分辨率，超过传感器像素大小（1.67μm）施加的理论奈奎斯特-香农采样分辨率限制的2.41倍。我们通过测量基准定量相位显微镜靶标，证实了该技术在QPI和分辨率增强方面的有效性。我们还表明，这种方法可以在培养箱中跟踪Hela细胞的生长，揭示大细胞群在较长时间内的细胞形态和亚细胞动力学。（2021）美国光学学会

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来源
《Optics Letters》 |2021年第9期|共4页
作者
Wu Xuejuan; Sun Jiasong; Zhang Jialin; Lu Linpeng; Chen Rong; Chen Qian; Zuo Chao;
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作者单位

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Jiangsu Key Lab Spectral Imaging &

Intelligent Se Nanjing 210094 Jiangsu Peoples R China;

Nanjing Univ Sci &

Technol Sch Elect &

Opt Engn Smart Computat Imaging Lab SCILab Nanjing 210094 Jiangsu Peoples R China;

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正文语种 eng
中图分类计量学;光学;
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7. Wide-field pathology imaging using on-chip microscopy [J] . Zhang Yibo, Greenbaum Alon, Luo Wei, Virchows Archiv: an international journal of pathology . 2015,第1期

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8. Lensfree On-Chip Fluorescence Microscopy for High-Throughput Imaging of Bio-Chips [C] . Ahmet F. Coskun, Serap Altay Arpali, Caglar Arpali, National Conference on Sensors . 2014

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9. Quantitative phase imaging microscopy with multi-wavelength optical phase unwrapping. [D] . Warnasooriya, Nilanthi. 2008

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Wavelength-scanning lensfree on-chip microscopy for wide-field pixel-super-resolved quantitative phase imaging

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