...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The FEBS journal >A guide to measuring phagosomal dynamics
【24h】

A guide to measuring phagosomal dynamics

机译：测量吞噬动态的指南

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phagocytosis is an essential mechanism for immunity and homeostasis, performed by a subset of cells known as phagocytes. Upon target engulfment,de novoformation of specialized compartments termed phagosomes takes place. Phagosomes then undergo a series of fusion and fission events as they interact with the endolysosomal system and other organelles, in a dynamic process known as phagosome maturation. Because phagocytes play a key role in tissue patrolling and immune surveillance, phagosome maturation is associated with signaling pathways that link phagocytosis to antigen presentation and the development of adaptive immune responses. In addition, and depending on the nature of the cargo, phagosome integrity may be compromised, triggering additional cellular mechanisms including inflammation and autophagy. Upon completion of maturation, phagosomes enter a recently described phase: phagosome resolution, where catabolites from degraded cargo are metabolized, phagosomes are resorbed, and vesicles of phagosomal origin are recycled. Finally, phagocytes return to homeostasis and become ready for a new round of phagocytosis. Altogether, phagosome maturation and resolution encompass a series of dynamic events and organelle crosstalk that can be measured by biochemical, imaging, photoluminescence, cytometric, and immune-based assays that will be described in this guide.
机译：吞噬作用是免疫和体内平衡的基本机制，由被称为吞噬细胞的细胞亚群执行。当目标物被吞噬后，会重新形成被称为吞噬体的特殊隔间。然后，吞噬体在与内溶酶体系统和其他细胞器相互作用的过程中经历一系列融合和分裂事件，这是一个称为吞噬体成熟的动态过程。由于吞噬细胞在组织巡逻和免疫监视中起着关键作用，因此吞噬体成熟与将吞噬作用与抗原呈递和适应性免疫反应的发展联系起来的信号通路有关。此外，根据货物的性质，吞噬体的完整性可能会受损，从而触发其他细胞机制，包括炎症和自噬。成熟完成后，吞噬体进入最近描述的阶段：吞噬体分解，降解货物的分解代谢物被代谢，吞噬体被再吸收，吞噬体来源的囊泡被回收。最后，吞噬细胞恢复体内平衡，为新一轮吞噬做好准备。总之，吞噬体成熟和分解包括一系列动态事件和细胞器串扰，可通过本指南中描述的生化、成像、光致发光、细胞仪和基于免疫的分析进行测量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号