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首页> 外文期刊>The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical >Modulating the Stability of Collagen Triple Helices by Terminal Charged Residues
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Modulating the Stability of Collagen Triple Helices by Terminal Charged Residues

机译：通过终端带电残留调节胶原三重螺旋的稳定性

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摘要

Cationic or anionic residues are frequently located at the termini of proteins because their charged side chain can form electrostatic interactions with a terminal carboxylate or ammonium group to stabilize the structure under physiological conditions. Here, we used collagen-mimetic peptides (CMPs) to examine how the terminal charge-charge interactions affect the collagen triple helix stability. We designed a series of CMPs with either a Lys or Glu incorporated into the terminus and measured their pH-dependent stability. The results showed that the terminal electrostatic attractions stabilized the triple helix, while the terminal electrostatic repulsions destabilized the trimer. The data also revealed that the repulsions imposed a greater effect than did the attractions on the triple helix. An amino acid with a shorter side chain, such as aspartate and ornithine, was also installed to investigate the length effect on electrostatic interactions, which was found to be insignificant. Meanwhile, simultaneously incorporating cationic and anionic residues into the termini showed slight additive stabilization effects but pronounced additive destabilization consequences. We have demonstrated that the collagen triple helix stability can be modulated by introducing a cationic or anionic residue into the terminus of a peptide, giving useful information for the design of collagen-associated materials.
机译：阳离子或阴离子残基通常位于蛋白质的末端，因为它们带电荷的侧链可以与末端羧酸盐或铵基形成静电相互作用，从而在生理条件下稳定结构。在这里，我们使用胶原模拟肽（CMPs）来研究末端电荷相互作用如何影响胶原三螺旋的稳定性。我们设计了一系列末端含有Lys或Glu的CMP，并测量了它们的pH依赖稳定性。结果表明，末端静电吸引稳定了三螺旋，而末端静电斥力使三聚体不稳定。数据还显示，斥力比吸引力对三螺旋线的影响更大。此外，还安装了一种侧链较短的氨基酸，如天冬氨酸和鸟氨酸，以研究静电相互作用的长度效应，这一效应被认为是无关紧要的。同时，在末端同时加入阳离子和阴离子残基显示出轻微的加性稳定效果，但显著的加性失稳后果。我们已经证明，胶原三螺旋的稳定性可以通过在肽的末端引入阳离子或阴离子残基来调节，这为胶原相关材料的设计提供了有用的信息。

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