...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >An Experimental Model of Human Recurrent Respiratory Papillomatosis: A Bridge to Clinical Insights
【24h】

An Experimental Model of Human Recurrent Respiratory Papillomatosis: A Bridge to Clinical Insights

机译：人类复发呼吸乳头瘤症的实验模型：临床见解桥梁

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objectives/Hypothesis To investigate the growth pattern of recurrent respiratory papilloma (RRP) implants on chicken embryo chorioallantoic membranes (CAMs) and to evaluate possible associations between the clinical course of the disease and the behavior of experimentally implanted RRP tumors. Study Design Experimental study. Methods Fresh 172 RRP tissue samples from 12 patients were implanted onto chick embryo CAMs. Morphological and morphometric analysis of the experimental CAM and chorionic epithelium was performed. The microvascular network of the CAM with the RRP implant was investigated under the effect of fluoresceinated anionic dextran. The peculiarities of the clinical course of the disease were evaluated. Results The implanted RRP tissue samples survived on CAMs in 86% of cases, retaining their essential morphologic characteristics and proliferative capacity of the original tumor. Implants induced thickening of both the CAM and the chorionic epithelium, but none of the RRP implants crossed the basement membrane of the hosting CAM. A "crawling film" of acellular material with newly formed papilloma sprouts located on the outer chorionic epithelium of the CAM was detected. Direct association between a recurrence rate of RRP and the number of newly formed papilloma sprouts around the implanted tumor on CAMs was revealed. Conclusion The chicken embryo CAM-based model is appropriate for investigations of RRP and facilitates the understanding of tumor biology and the clinical course of the disease, thus providing the basis for further research and acceleration of the identification and development of new specific therapeutic compounds that limit the spread and recurrence of RRP. Level of Evidence N/ALaryngoscope, 2020
机译：目的/假设：研究复发性呼吸道乳头状瘤（RRP）植入鸡胚绒毛尿囊膜（CAMs）的生长模式，并评估疾病的临床病程与实验植入的RRP肿瘤行为之间的可能关联。研究设计实验研究。方法将12例患者的172个新鲜RRP组织植入鸡胚CAMs。对实验性CAM和绒毛膜上皮进行形态学和形态计量学分析。在荧光阴离子右旋糖酐的作用下，研究了带有RRP植入物的CAM的微血管网络。评估了该病临床病程的特点。结果86%的患者植入的RRP组织样本在CAMs上存活，保留了原始肿瘤的基本形态特征和增殖能力。植入物诱导CAM和绒毛膜上皮增厚，但RRP植入物均未穿过宿主CAM的基底膜。在CAM的外绒毛膜上皮上发现了一层无细胞材料的“爬行膜”，其中含有新形成的乳头状瘤芽。RRP复发率与CAMs植入肿瘤周围新形成的乳头状瘤芽数之间存在直接联系。结论基于鸡胚CAM的模型适用于RRP的研究，有助于理解肿瘤生物学和疾病的临床过程，从而为进一步研究和加速鉴定和开发限制RRP传播和复发的新的特异性治疗化合物提供基础。证据级别N/ALaryngoscope，2020年

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号