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首页> 外文期刊>The Journal of Experimental Biology >Effects of variable oxygen regimes on mitochondrial bioenergetics and reactive oxygen species production in a marine bivalve, Mya arenaria
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Effects of variable oxygen regimes on mitochondrial bioenergetics and reactive oxygen species production in a marine bivalve, Mya arenaria

机译：可变氧体制对海洋双嗜酸盐术中线粒体生物植物和反应性氧物种生产的影响

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摘要

Estuarine and coastal benthic organisms often experience fluctuations in oxygen levels that can negatively impact their mitochondrial function and aerobic metabolism. To study these impacts, we exposed a common sediment-dwelling bivalve, the soft-shell clam Mya arenaria, for 21 days to chronic hypoxia (P-O2 similar to 4.1 kPa), cyclic hypoxia (P-O2 similar to 12.7-1.9 kPa, mean 5.7 kPa) or normoxia (P-O2 similar to 21.1 kPa). pH was manipulated to mimic the covariation in CO2/pH and oxygen levels in coastal hypoxic zones. Mitochondria' respiration, including proton leak, the capacity for oxidative phosphorylation (OXPHOS), the maximum activity of the electron transport system (ETS), reactive oxygen species (ROS) production, and activity and oxygen affinity of cytochrome c oxidase (CCO) were assessed. Acclimation to constant hypoxia did not affect the studied mitochondrial traits except for a modest decrease in the OXPHOS coupling efficiency. Cyclic hypoxia had no effect on OXPHOS or ETS capacity, but increased proton leak and lowered mitochondrial OXPHOS coupling efficiency. Furthermore, mitochondria of clams acclimated to cyclic hypoxia had higher rates of ROS generation compared with the clams acclimated to normoxia or chronic hypoxia. CCO activity was upregulated under cyclic hypoxia, but oxygen affinity of CCO did not change. These findings indicate that long-term cyclic hypoxia has a stronger impact on the mitochondria of M. arenaria than chronic hypoxia and might lead to impaired ATP synthesis, higher costs of mitochondrial maintenance and oxidative stress. These changes might negatively affect populations of M. arenaria in the coastal Baltic Sea under increasing hypoxia pressure.
机译：河口和沿海底栖生物经常经历氧气水平的波动，这会对其线粒体功能和有氧代谢产生负面影响。为了研究这些影响，我们将一种常见的沉淀物双壳贝类，即软壳蛤蜊暴露在慢性缺氧（P-O2类似于4.1 kPa）、周期性缺氧（P-O2类似于12.7-1.9 kPa，平均5.7 kPa）或常氧（P-O2类似于21.1 kPa）下21天。控制pH值，以模拟沿海缺氧区CO2/pH和氧气水平的协变量。评估线粒体的呼吸，包括质子泄漏、氧化磷酸化能力（OXPHOS）、电子传递系统（ETS）的最大活性、活性氧（ROS）的产生以及细胞色素c氧化酶（CCO）的活性和氧亲和力。除了OXPHOS偶联效率略有降低外，对持续缺氧的适应对所研究的线粒体特征没有影响。循环缺氧对氧磷或ETS容量没有影响，但增加了质子泄漏，降低了线粒体氧磷偶联效率。此外，与适应常氧或慢性缺氧的蛤蜊相比，适应周期性缺氧的蛤蜊线粒体的ROS生成率更高。循环缺氧时CCO活性上调，但CCO的氧亲和力没有改变。这些发现表明，与慢性缺氧相比，长期周期性缺氧对沙芥线粒体的影响更大，并可能导致ATP合成受损、线粒体维持成本增加和氧化应激。在不断增加的缺氧压力下，这些变化可能会对波罗的海沿岸的沙芥种群产生负面影响。

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