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首页> 外文期刊>The Journal of Experimental Biology >Effects of Na+ channel isoforms and cellular environment on temperature tolerance of cardiac Na+ current in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss)
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Effects of Na+ channel isoforms and cellular environment on temperature tolerance of cardiac Na+ current in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss)

机译：Na +通道同种型和细胞环境对斑马鱼（Danio Rerio）和彩虹鳟鱼心脏Na +电流耐热性的影响（Oncorhynchus mykiss）

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Heat tolerance of heart rate in fish is suggested to be limited by impaired electrical excitation of the ventricle due to the antagonistic effects of high temperature on Na+(l(Na)) and K+. (l(K1)) ion currents (l(Na) is depressed at high temperatures while lK(1) is resistant to them). To examine the role of Na+ channel proteins in heat tolerance of l(Na), we compared temperature dependencies of zebrafish (Danio redo, warm-dwelling subtropical species) and rainbow trout (Oncorhynchus mykiss, cold-active temperate species) ventricular l(Na) and l(Na) generated by the cloned zebrafish and rainbow trout Na(v)1.4 and Na(v)1.5 Na+ channels in human embryonic kidney (HEK) cells. Whole-cell patch-clamp recordings showed that zebrafish ventricular l(Na) has better heat tolerance and slower inactivation kinetics than rainbow trout ventricular l(Na). In contrast, heat tolerance and inactivation kinetics of zebrafish and rainbow trout Na(v)1.4 channels are similar when expressed in the identical cellular environment of HEK cells. The same applies to Na(v)1.5 channels. These findings indicate that thermal adaptation of ventricular l(Na) is largely achieved by differential expression of Na+ channel alpha subunits: zebrafish that tolerate higher temperatures mainly express the slower Na(v)1.5 isoform, while rainbow trout that prefer cold waters mainly express the faster Na(v)1.4 isoform. Differences in elasticity (stiffness) of the lipid bilayer and/or accessory protein subunits of the channel assembly may also be involved in thermal adaptation of l(Na). The results are consistent with the hypothesis that slow Na+ channel kinetics are associated with increased heat tolerance of cardiac excitation.
机译：由于高温对Na+（l（Na））和K+的拮抗作用，鱼类心率的耐热性受到心室电兴奋受损的限制。（l（K1））离子电流（l（Na）在高温下被抑制，而lK（1）对它们有抵抗力）。为了研究Na+通道蛋白在l（Na）耐热性中的作用，我们比较了斑马鱼（Danio redo，温暖居住的亚热带物种）和虹鳟鱼（Oncorhynchus mykiss，寒冷活跃的温带物种）心室l（Na）和l（Na）在人类胚胎肾（HEK）细胞中克隆的斑马鱼和虹鳟鱼Na（v）1.4和Na（v）1.5 Na+通道产生的温度依赖性。全细胞膜片钳记录显示，斑马鱼心室l（Na）比虹鳟心室l（Na）具有更好的耐热性和较慢的失活动力学。相比之下，当在HEK细胞的相同细胞环境中表达时，斑马鱼和虹鳟Na（v）1.4通道的耐热性和失活动力学相似。这同样适用于Na（v）1.5通道。这些发现表明，心室l（Na）的热适应在很大程度上是通过Na+通道α亚单位的差异表达实现的：耐高温的斑马鱼主要表达较慢的Na（v）1.5亚型，而喜欢冷水的虹鳟鱼主要表达较快的Na（v）1.4亚型。脂质双层和/或通道组件的辅助蛋白亚单位的弹性（刚度）差异也可能与l（Na）的热适应有关。这一结果与缓慢的钠通道动力学与心脏兴奋的耐热性增加相关的假设一致。

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