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首页> 外文期刊>The Journal of Experimental Biology >Characterization of Halyomorpha halys TAR1 reveals its involvement in (E)-2-decenal pheromone perception
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Characterization of Halyomorpha halys TAR1 reveals its involvement in (E)-2-decenal pheromone perception

机译：卤代素卤莽的表征Tar1揭示其参与（e）-2-渐象的信息素感知

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In insects, tyramine receptor 1 (TAR1) has been shown to control several physiological functions, including olfaction. We investigated the molecular and functional profile of the Halyomorpha halys type 1 tyramine receptor gene (HhTAR1) and its role in olfactory functions of this pest. Molecular and pharmacological analyses confirmed that the HhTAR1 gene codes for a true TAR1. RT-qPCR analysis revealed that HhTAR1 is expressed mostly in adult brain and antennae as well as in early development stages (eggs, 1st and 2nd instar nymphs). In particular, among the antennomeres that compose a typical H. halys antenna, HhTAR1 was more expressed in flagellomeres. Scanning electron microscopy investigation revealed the type and distribution of sensilla on adult H. halys antennae: both flagellomeres appear rich in trichoid and grooved sensilla, known to be associated with olfactory functions. Through an RNAi approach, topically delivered HhTAR1 dsRNA induced a 50% downregulation in gene expression after 24 h in H. halys 2nd instar nymphs. An innovative behavioural assay revealed that HhTAR1 RNAi-silenced 2nd instar nymphs were less susceptible to the alarm pheromone component (E)-2 decenal as compared with controls. These results provide critical information concerning the role of TAR1 in olfaction regulation, especially alarm pheromone reception, in H. halys. Furthermore, considering the emerging role of TAR1 as target of biopesticides, this work opens the way for further investigation on innovative methods for controlling H. halys.
机译：在昆虫中，酪胺受体1（TAR1）已被证明可以控制多种生理功能，包括嗅觉。我们研究了Halyomorpha halys 1型酪胺受体基因（HhTAR1）的分子和功能特征及其在这种害虫嗅觉功能中的作用。分子和药理学分析证实HhTAR1基因编码真正的TAR1。RT-qPCR分析显示，HhTAR1主要在成人大脑和触角以及早期发育阶段（卵、1龄和2龄若虫）表达。特别是，在构成典型H.halys触角的触角名称中，HhTAR1更多地在鞭毛中表达。扫描电子显微镜研究揭示了成年哈雷鱼触角上感受器的类型和分布：这两种鞭毛均富含毛状和沟槽状感受器，已知与嗅觉功能有关。通过RNAi方法，局部递送的HhTAR1 dsRNA在h.halys 2龄若虫中诱导了24小时后50%的基因表达下调。一项创新的行为分析显示，与对照组相比，HhTAR1 RNA干扰沉默的2龄若虫对报警信息素成分（E）-2 decental不太敏感。这些结果提供了有关TAR1在H.halys嗅觉调节，尤其是警报信息素接收中作用的关键信息。此外，考虑到TAR1作为生物杀虫剂靶标的新作用，这项工作为进一步研究控制H.halys的创新方法开辟了道路。

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