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首页> 外文期刊>Dalton transactions: An international journal of inorganic chemistry >Metal-enhanced sensing platform for the highly sensitive detection of C-reactive protein antibody and rhodamine B with applications in cardiovascular diseases and food safety
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Metal-enhanced sensing platform for the highly sensitive detection of C-reactive protein antibody and rhodamine B with applications in cardiovascular diseases and food safety

机译：金属增强型传感平台，用于C反应蛋白抗体和罗丹明B的高敏感检测，具有心血管疾病和食品安全的应用

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The potential applications of metal-enhanced fluorescence (MEF) devices include biosensors for the detection of trace amounts in biosciences, biotechnology, and pathogens that are relevant to medical diagnostics and food control. In the present study, the silver (Ag) film thickness (56 nm) of an MEF system was calibrated to maximize the depth-to-width ratio (Gamma) of the surface plasmon resonance (SPR) active metal from reflectance dip curves. Upon plasmon coupling with thermally evaporated Ag, we demonstrated a 2.21-fold enhancement compared to the pristine flat substrate with the coefficient of variation (CV) approximate to 0.22% and the limit of detection (LOD) 0.001 mg L-1 of the concentration of an Alexa Fluor 488-labeled anti-C-reactive protein antibody (CRP@Alexa fluor 488). The structure was developed to simplify the in situ generation of biosensors for the surface-enhanced Raman spectroscopy (SERS) to determine Rhodamine B (RhB) with a highly robust performance. The procedure presented a simple and rapid sample pretreatment for the determination of RhB with a limit of quantification of 10(-10) M and a satisfactory linear response (0.98). The results showed the excellent performance of the surface plasmon coupled emission (SPCE), which opens up possibilities for the accurate detection of small-volume and low-concentration target analytes due to the improved sensitivity and signal-to-noise ratio (SNR).
机译：金属增强荧光（MEF）设备的潜在应用包括生物传感器，用于检测与医疗诊断和食品控制相关的生物科学、生物技术和病原体中的痕量。在本研究中，对MEF系统的银（Ag）膜厚度（56 nm）进行了校准，以根据反射倾角曲线最大化表面等离子体共振（SPR）活性金属的深宽比（γ）。在等离子体激元与热蒸发Ag偶联后，我们证明，与原始平坦基质相比，变异系数（CV）约为0.22%，检测限（LOD）为0.001 mg L-1的Alexa Fluor 488标记的抗C反应蛋白抗体浓度增加了2.21倍(CRP@Alexa福陆488）。该结构的开发旨在简化用于表面增强拉曼光谱（SERS）的生物传感器的原位生成，从而以高度鲁棒的性能测定罗丹明B（RhB）。该程序提供了一种简单、快速的RhB测定样品预处理方法，定量限为10（-10）M，线性响应令人满意（0.98）。结果表明，表面等离子体激元耦合发射（SPCE）具有优异的性能，由于灵敏度和信噪比（SNR）的提高，为小体积、低浓度目标分析物的准确检测开辟了可能性。

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